Prevalence of Class I and II Integrons among MDR Enterobacter cloacae Isolates Obtained from Clinical Samples of Children in Kermanshah, Iran
Journal Title: Journal of Clinical and Diagnostic Research - Year 2018, Vol 12, Issue 12
Abstract
ABSTR ACT Introduction: Enterobacter species are among one of the key causes of hospital infections. The transfer of drug resistance genes through the integrons promotes the development of antibiotic resistance and the emergence of Multidrug Resistant (MDR) strains. Aim: The aim of this study was to determine the prevalence of class I and II Integrons among MDR Enterobacter cloacae isolates obtained from clinical samples of children in Kermanshah, Iran. Materials and Methods: This descriptive cross-sectional study was done during 11 month period from October 2016 to September 2017, 72 isolates of E.cloacae were collected from children under 15 years of age in Mohammad Kermanshahi Hospital in Kermanshah, Iran. After confirmation of the isolates with biochemical specific tests, their antibiotic susceptibility with disk diffusion was examined. Then the frequency of Class I and II integrons was determined by using their specific primers and by PCR method. Data was analysed by SPSS software version 20 and p-values less than 0.05 were considered statistically significant. Results: The highest and lowest frequency of isolates were in blood samples 29 (40.3%) and CSF 2 (2.8%) respectively. From the 72 isolates of E.cloacae, 54 isolates (75%) were MDR. The highest antibiotic resistance was observed against Ampicillin-clavulanic acid (94.4%) and Cefalexin (69.4%), whereas the lowest antibiotic resistance was to Imipenem (9.7%) and Colistin (6.9%). Genotypically, the frequency of class I integron was 42 (58.3%), but none of the isolates had class II integron. Conclusion: The results of this study demonstrates that E.cloacae isolated from children, in addition to the high frequency of MDR isolates, the prevalence of isolates with integron is expanding. Therefore, keeping with the role of integrons in resistance to different antibiotics, it is necessary to pay greater attention to identify them.
Authors and Affiliations
Seyed Hamidreza Mortazavi, Faizullah Mansouri, Mohsen Azizi, Amirhooshang Alvandi, Ali Karbasfrushan, Nahid Madadi-Goli, Sepideh Fereshteh, Kamal Ahmadi
Keywords
Related Articles
- EP ID EP525844
- DOI 10.7860/JCDR/2018/37826.12396
- Views 52
- Downloads 0
Diode Laser-Assisted Fibrotomy in the Management of Oral Sub-Mucous Fibrosis: A New Technique in Surgical Management
Oral Sub-Mucous Fibrosis (OSMF) has a high prevalence in the Indian subcontinent and is a matter of grave concern because of its alarming malignant potential. Numerous treatment modalities; from antioxidants, vitamin sup...
Catalysing Public Healthcare Efficacy: Striking the Right Cords of Leadership
Abstract Introduction: Leaders’ behaviour significantly influences the job satisfaction of the employees. Hospital experience and patient outcomes relate to job satisfaction of employee’s working therein. Aim: Current...
Successful Angioplasty of Left Vertebral Artery and Right Subclavian Artery Via Retrograde Approach
We describe a 77-year-old male who had right upper limb ischemic symptoms and history of unsuccessful right subclavian artery angioplasty. According to ultrasound findings, upper limb angiography was performed which conf...
Surrogate Anthropometric Parameters for Assessment of Low Birth Weight Babies
ABSTRACT Introduction: Low Birth Weight (LBW) accounts for significant proportion of infants and under five mortality. Screening of LBW babies in peripheral areas with poor facilities, with surrogate anthropometric param...
Role of Ultrasonography and Ultrasound Elastography in the Evaluation of Thyroid Nodules
ABSTRACT Introduction: Thyroid nodules are frequently encountered with its Incidence is increasing steadily. Most nodules are benign and <5% of nodules are malignant. High-Resolution Ultrasonography (HRUS) is the most se...