Prevalence of extended β lactamases, Amp C and metallo beta lactamases among gram negative clinical isolates at a tertiary care centre in South India
Journal Title: Medpulse International Journal of Microbiology - Year 2018, Vol 7, Issue 2
Abstract
Background: Resistance of Gram negative bacteria to broad spectrum antibiotics is posing a clinical threat during the last decade. These ESBL and MBL producing organisms have a potential for rapid dissemination and hence their early detection is necessary to establish appropriate antibiotic therapy. Aims: This study aims to determine the prevalence of ESBL, AmpC and MBL producers among gram negative clinical isolates. Material and Methods: Four hundred and seventy four gram negative bacilli were isolated from various clinical specimens. Sample processing and identification was done by standard microbiological techniques. The antibiotic sensitivity test was performed and all the gram negative isolates were tested for ESBL by DDST and PCDDT method, AmpC and MBL production. Observation and Results: 474 gram negative bacilli were isolated from various clinical samples and the commonest isolate was E.coli (40.2%), Klebsiella pneumoniae (21.5%), Pseudomonas aeruginosa (19.6%). ESBL detection rate was found more by PCDDT. AmpC production was seen in Acinetobacter (14.8%), Enterobacter spp. (11.1%), Pseudomonas (9.6%.) Majority of MBL producers were Pseudomonas aeruginosa (18.2%) and cinetobacter (17%). Conclusion: Incidence of β lactamases producing enzymes is tremendously increasing hence laboratory detection of these organisms producing ESBL, AmpC and MBL is becoming more important. Proper infection control practices should be adopted to curtail the spread of infections produced by organisms producing beta lactamases enzymes.
Authors and Affiliations
Purti C Tripathi, Trinain Kumar Chakraverti
Keywords
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