Anti-Mycobacterial activity of selected medicinal plants
Journal Title: Medpulse International Journal of Microbiology - Year 2017, Vol 4, Issue 3
Abstract
Background: In recent past Mycobacterium developed resistance against both the first line as also the second line drugs and there is emergence of multi-drug resistant (MDR) and extensively-drug resistant (XDR) strains of M. tuberculosis all over the world including India. In addition to developing resistance, Anti-TB drug induced hepatotoxicity, which is a common serious adverse drug reaction, is one of the most challenging clinical problems. India is one of the few countries in the world which has unique wealth of medicinal plants and vast traditional knowledge of use of herbal medicine for cure of various diseases. Therefore, the present study was carried out to check the anti-mycobacterial activity of extracts of various plants viz. Lantana camara and Ocimum sanctum against standard strain of M. avium. Methods: Water and Methanolic extracts at concentration of 2%, 4% and 6% of both pants were tested in vitro for their anti-mycobacterial activity against M. avium using Lowenstein-Jensen (L J) medium. Percentage inhibition was calculated by mean reduction in number of colonies on extracts containing media as compared to extract free (control) media. Results: Out of two extracts of both the plants Lantana camara and Ocimum sanctum, all four exhibited the inhibitory activity against M. avium. At 6% concentration of extract in L J Medium the percentage inhibition of M avium for Lantana camara was 63 and 67 for water and Methanolic extract respectively. For same concentration water and Methanolic extract of Ocimum sanctum showed 71 and 75 percent inhibition of M avium. Interpretations and Conclusions: Our study has shown positive results regarding anti-mycobacterial activity of studied plants. Further studies need to be carried out using different solvents or using fractions of crude extracts to finally conclude the potential of these plants as an anti-mycobacterial treatment.
Authors and Affiliations
Geeta Parihar, Rasik Bihari Vyas, Priyanka Soni, Jyotsna Chandwani, Manoj Sharma
Keywords
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