Speciation and resistotyping of coagulase negative staphylococci from clinical samples in a tertiary care hospital
Journal Title: Medpulse International Journal of Microbiology - Year 2017, Vol 4, Issue 3
Abstract
Background: Coagulase negative Staphylococci (CONS) are the indigenous flora of the human skin and mucous membrane. They have long been considered as non-pathogenic and were rarely reported to cause severe infections. However, as a result of the combination of increased use of Intravascular devices and an increase in the number of hospitalised immunocompromised patients, CONS have become the major cause of Nosocomial blood stream infections and they account for 9% of all nosocomial infections. These infections are difficult to treat because of the multiple drug resistant nature of the organisms. Objective: Isolation, identification and speciation of coagulase negative Staphylococci from various clinical specimens and study their antimicrobial susceptibility pattern. Material and Methods: Out of 1280 samples collected for this study, 100 strains of CONS were isolated from exudates, urine and blood of clinically diagnosed cases by standard identification methods. Antibiotic susceptibility testing of the isolated strain was carried out by Kirby-Bauer disc diffusion method. Results: The most common CONS species isolated were S. epidermidis 59 (59%), followed by S. saprophyticus 29 (29%), S. haemolyticus 9 (9%), S. xylosus 2 (2%) and S. capitis 1 (1%). Maximum number of S. epidermidis were isolated from exudates 36 (72%) and S. saprophyticus from urine 23 (74.19%). The antibiotic susceptibility testing showed, Vancomycin as the most effective drug followed by Ciprofloxacin. Methicillin resistant CONS were 38 (38 %). Conclusion: The most common species isolated was S.epidermidis, which indicates it is a pathogen of significance and not merely a commensal. S.saprophyticus was the most common urinary isolate which indicates that this is a very common urinary pathogen. The antibiotic resistance pattern in CONS is a great threat to the clinicians and the widespread occurrence of methicillin resistance poses a great problem, which needs special attention and intensive study.
Authors and Affiliations
Qader Ahmed Jalily, Ganesh Oruganti
Keywords
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- EP ID EP487287
- DOI 10.26611/1008431
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