Detection of prevalence of antinuclear antibodies in clinically suspected cases of systemic autoimmune diseases by indirect immunofluorescence test in a tertiary care hospital
Journal Title: Medpulse International Journal of Microbiology - Year 2019, Vol 9, Issue 2
Abstract
Background: Systemic autoimmune diseases (SAD) are the diseases where multiple organs are involved in the presence of auto antibodies directed against sub-cellular structures or molecules and are characterized by presence of Antinuclear antibodies (ANA). Indirect immunofluorescence test (IIFA) on Hep-2 (human epithelial cell tumour line) is a “gold standard” technique for detection of ANA Purpose: 1) To detect the Prevalence of Antinuclear antibodies (ANA) in clinically suspected cases of Systemic autoimmune diseases (SAD) by Indirect Immunofluorescence test(IIFA)on Hep2 cells Methodology: A total of 150 clinically suspected cases of SAD of both sexes and above 18yrs of age from various departments were included in the study and blood samples collected were subjected to Indirect Immunofluorescence test on Hep-2 cells coated slides and slides were visualized in fluorescent microscope using blue green filter (450nm). Results: 150 samples were analysed for ANA by IIFA. Out of 150 samples, 54 samples were positive by IIFA. Prevalence of ANA among clinically suspected case of SAD by IIFA was found to be 36%. Female predominance was seen and most common age group of presentation was 20-30yrs. The homogenous pattern was the most common (n=26; 48.14%) pattern followed by speckled pattern (n = 19; 35%). Conclusions: Systemic autoimmune disorders are chronic conditions with no cure and are growing day by day. With the increase in prevalence of systemic autoimmune diseases there is a need for early and accurate diagnostic techniques. Therefore, early detection of ANA by Gold Standard Indirect Immunofluorescence test in a Clinically suspected cases helps to reduce disease morbidity and mortality
Authors and Affiliations
Jabeen Begum, V V Shailaja, Rajeshwar Rao
Keywords
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- EP ID EP486265
- DOI 10.26611/1008925
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