Prevalence of Pseudomonas aeruginosa isolated from clinical specimen with reference to biofilm formation and detection of metallo-β-lactamase in a tertiary care rural hospital.
Journal Title: International Journal of Medical Science And Advanced Clinical Research (IJMACR) - Year 2019, Vol 2, Issue 3
Abstract
Background: Pseudomonas aeruginosa is aerobic, gram-negative bacilli. It can survive with low levels of nutrients and grow in temperatures ranging from 4-42°C and has an ability to colonized many natural and artificial environments and has important property of biofilm formation. It is a paragon of opportunistic nosocomial pathogen, fabled for its multidrug resistance (MDR) and critical life threatening infections. Objective: To determine prevalence, biofilm production and antibiotic susceptibility pattern of P.aeruginosa isolated from clinically significant samples. Material And Method: The cross sectional study was carried for period of 3 month(ICMRSTS) in the microbiology department and included total of 100 P.aeruginosa isolated from clinically significant samples. These isolates were identified by standard procedures and were tested for phenotypic detection of biofilm formation and antibiotic resistance pattern. Result: Maximum prevalence was seen in age group below 20 years(31 isolates), in males(75 isolates) and specimen wise in pus sample(30 isolates),department wise from surgery department(32 isolates). 81 isolates showed biofilm production by standardtube method. P.aeruginosa was highly resistant to Ceftazidime(74%) and least resistant to colistin(2%). 34% strains were MBL producers, 19% strains were AMPc producer, and both AMPc and MBL production were seen in 87 isolates and 56 were MDR P.aeruginosa. Conclusion: Present study showed high prevalence of biofilm production and antibiotic resistance in P aeruginosa with MBL and AMPc production. Hence it is necessary to have regular surveillance of drug resistance in P aeruginosa which will be useful for selecting an appropriate antibiotic to know changing trends in antibiotic susceptibility pattern and for limiting the use of colistin and save it for the treatment of resistant and life threatening P. aeruginosa.
Authors and Affiliations
Dr. Suvarna Sande (Tathe)
Keywords
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