Process Optimization for Production of Bovine Bone Collagenase by B.cereus MBL13-U

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Process Optimization for Production of Bovine Bone Collagenase by B.cereus MBL13-U

Journal Title: 河南科技大学学报（自然科学版） - Year 2018, Vol 39, Issue 1

Abstract

To obtain the new collagenase which could efficiently degrade bovine bone collagen, the Bacillus cereus MBL13-U was used as the starting strain, and the collagenase activity was used as an indicator.The single factor experiment was performed on four factors (inoculation amount of strain, fermentation temperature, initial p H, and fermentation time) , respectively.Through four-factor quadric orthogonal rotation experiment, the optimum process of fermentation for production of bovine bone collagenase by B.cereus MBL13-U was acquired.When inoculation amount of strain was 4% (volume fraction) , fermentation temperature was 35 ℃, initial p H was 6.4 and fermentation time was 46 h, the activity of collagenase was (92.31 ± 1.13) U/mL.Then bovine bone collagen was disposed by the collagenase.The structural properties of bovine bone collagen in enzymolysis were characterized by scanning electron microscope.The results show that the intact surface structure of bovine bone collagen is destroyed by the enzymolysis, so the type I collagen is more exposed to the surface and the hydrolysis is faster.

Authors and Affiliations

Lili LIU, Dan LI, Chenliu YANG, Yanyu LIANG, Yu LI

Keywords

Bacillus cereus MBL13-U; fermentation; collagenase; optimization; bovine bone collagen;

EP ID EP464635
DOI -
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