Production and Stability Studies of the Biosurfactant Isolated from Alkaliphilic Bacterium SJS1
Journal Title: Bio-Science Research Bulletin - Year 2018, Vol 34, Issue 1
Abstract
Background: Mostly oil spills occurs in sea ocean and coastal areas, required the best candidate for the degradation of hydrocarbons such as chemical surfactant and synthetic chemicals to minimize such oil spills are primarily effective strategies with environmental risk so the aims of these studies the isolation and characterization of a alkaline and halotolerant bacteria which was isolated from Lonar Crater and able to utilize different oil as carbon source and produces biosurfactant. Methodology: In present investigation was to determine surfactant substance producing bacterium of Lonar Lake using minimal salt medium with various oil as a carbon sources. Biosurfactant-producing microorganisms were isolated and screening was done on the basis of Drop collapse test, Oil spread and emulsification index. Result: A total of six bacteria were isolated from the water and sediment samples collected from the of Lonar crater, India. Out of them SJS1was selected for production and, partial characterizations of biosurfactant. A Gram negative bacterium was studied by morphological, physiological and biochemical characterization. The bacterium SJS1 grew in medium containing sodium chloride (NaCl w/v) from 0.5 to 7% and at pH 7–12. The production of a biosurfactant by Bacteria SJS1 was studied to evaluate the influence of the concentration of different oil. SJS1 was able to grow at high salinity conditions and produce biosurfactants. The organism grew and produced biosurfactant when cultured in salinities up to 3 g l-1and temperatures up to 60°C. The biosurfactant was highly stable over broad temperature, pH and NaCl, showing excellent thermostablity, and haloalkaline tolerant nature. The biosurfactant produced by the organism emulsified a range of oil with as Ground nut and Coconut oil best substrate whereas Sesame oil was the poorest. Interpretation: This is valuable information for biosurfactant production and optimization has bright future towards the improvement and production of novel biosurfactant for entirely new areas of environmental and biotechnological applications. The results confirmed, their enhancing capability on both efficiency and rate of hydrocarbon degradation from water and soil.
Authors and Affiliations
Dr. Vishal Ramakant Dhundale
Keywords
Related Articles
- EP ID EP528879
- DOI 10.5958/2320-3161.2018.00001.9
- Views 157
- Downloads 0
Enhancement of Growth and Medicinal Potential of Senna alata (L.) Roxb. Through the Application of Organic Manures
The importance of medicinal plants in the healthcare delivery of man cannot be overemphasized. Enhancing optimum production and improving the performances of these plants both quantitatively and qualitatively is therefor...
Major Concerns with the Cryostorage of Mammalian Cell Cultures
Cell cultures are of 3 types, viz. animal/human, bacterial and plant. In the laboratory, storage protocols are used to keep, preserve or regenerate these cells. However, the situation in all 3 cell types, with regard to...
Role of Nanotechnology in Food Sector
Nanotechnology is a new emerging technology which has applications in different areas like it plays an important role in food sector and is used during food processing and food packaging. Nano-food may provide proper nut...
Ecofriendly Management of Paddy Crop Residues for Sustainable Environment and Development
The world-wide mechanization leads to large amount of rice crop residue or stubbles production. The management of rice (paddy) crop’s post harvested residue is done by burning, incorporation/ amalgamation, surface retent...
Production and Stability Studies of the Biosurfactant Isolated from Alkaliphilic Bacterium SJS1
Background: Mostly oil spills occurs in sea ocean and coastal areas, required the best candidate for the degradation of hydrocarbons such as chemical surfactant and synthetic chemicals to minimize such oil spills are pri...