Production, Characterization and Valuable Applications of Exopolysaccharides from Marine Bacillus subtilis SH1
Journal Title: Polish Journal of Microbiology - Year 2017, Vol 66, Issue 4
Abstract
Exopolysaccharides (EPSs) are high molecular weight polymers consisting of different sugar residues they are preferable for replacing synthetic polymers as they are degradable and nontoxic. Many microorganisms possess the ability to synthesize and excrete exopolysaccharides with novel chemical compositions, properties and structures to have potential applications in different fields. The present study attempt to optimize the production of EPS by marine Bacillus subtilis SH1 in addition to characterization and investigation of different valuable applications. Effect of medium type, incubation period and pH were studied using the one factor at a time experiments. It was shown that the highest productivity (24 gl–1) of exopolysaccharides was recorded by using yeast malt glucose medium with pH 9 at the fourth day of incubation. Experimental design using Response Surface Methodology (RSM) was applied to optimize various nutrients at different concentrations. The finalized optimized medium contained (gl–1) glucose (5), peptone (2.5), yeast extract (4.5) and malt extract (4.5) increased the production of EPS to 33.8 gl–1 with1.4 fold increase compared to the basal medium. Chemical characterization of the extracted EPS showed that, FTIR spectra exhibited bands at various regions. Moreover, HPLC chromatogram indicated that the EPS was a heteropolysaccharide consisting of maltose and rhamnose. The study was extended to evaluate the potentiality of the extracted polysaccharides in different medical applications. Results concluded that, EPS exhibited antibacterial activity against Aeromonas hydrophila, Pseudomonas aeruginosa and Streptococcus faecalis and the highest antibacterial activity (7.8, 9 and 10.4 AU/ml) was against S. faecalis at 50, 100 and 200 mg/ml respectively. The EPS exhibited various degree of antitumor effect toward the tested cell lines (MCF-7, HCT-116 and HepG2). In addition, EPS exhibited antiviral activity at 500 μg/ml. The antioxidant capacity increased with increasing the concentration of the sample. Scanning electron microscopic analysis showed that EPS had compact film-like structure, which could make it a useful in the future applications as in preparing plasticized film.
Authors and Affiliations
Sahar W. M. Hassan, Hassan A. H. Ibrahim
Keywords
Related Articles
- EP ID EP244328
- DOI 10.5604/01.3001.0010.7001
- Views 92
- Downloads 0
<strong>Relationship between</strong><strong> measles outbreaks based on genetic analysis of measles virus genomes detected in patients in Poland between 2006 and 2012.</strong>T
This study describes the molecular characterization of 56 MeV strains obtained from clinical samples collected from 56 patients in Poland from 2006 to 2012. MeV RNA samples were analysed according to variability of C-ter...
Effect of DNA Extraction Methods on the Apparent Structure of Yak Rumen Microbial Communities as Revealed by 16S rDNA Sequencing
To better identify the microbial community of yak rumen, the standardization of DNA extraction is key to ensuring fidelity while studying environmental microbial communities. In this study, we systematically compared the...
Evaluation of Two Lactic Acid Bacteria Starter Cultures for the Fermentation of Natural Black Table Olives ([i]Olea europaea[/i] L cv Kalamon)
The production of Greek-style natural black table olives remains an empirical process relying on spontaneous fermentation despite its economic significance. For this reason producers often resort to increased NaCl c...
The probable interspecies transfer of the bla<sub>VIM-4</sub> gene between Enterobacter cloacae and Klebsiella pneumoniae in a single infant patient
We report interspecies transfer of the [i]bla[/i][sub]VIM-4[/sub] gene in MBL-producing [i]Enterobacter cloacae[/i] and Klebsiella pneumoniae isolates from a newborn patient who had received meropenem therapy. We sh...
Microbial Products and Biofertilizers in Improving Growth and Productivity of Apple – a Review
The excessive use of mineral fertilizers causes many negative consequences for the environment as well as potentially dangerous effects of chemical residues in plant tissues on the health of human and animal consumers. B...