PRODUCTION OF α-AMYLASE FROM ASPERGILLUS FLAVUS UNDER SOLID STATE FERMENTATION WITH OPTIMUM CONDITIONS
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2012, Vol 3, Issue 8
Abstract
α-amylases are ubiquitous enzymes produced by plants, animals and microbes, where they play a dominant role in carbohydrate metabolism. In spite of the wide distribution of amylases, microbial sources, namely fungal and bacterial amylases, are used for the industrial production due to advantages such as cost effectiveness, consistency, less time and space required for production and ease of process modification and optimization. Filamentous fungi are known to be prolific producers of extracellular proteins; they are widely exploited for the production of different enzymes including a-amylase. SSF is generally defined as the growth of microorganisms on moist solid substrates with negligible free water, which is preferred than SmF because of simple technique, low capital investment, lower levels of catabolite repression, end- product inhibition, low waste water output, better product recovery and high quality production. Different substrates such as wheat bran, mollasses bran, rice bran, maize meal and sugarcane bagasse were used; out of which sugarcane bagasse has been reported to produce promising results. Different carbon and nitrogen supplements were given which increased the yield of the enzyme and optimized the pH as 6.0 and temperature 300C. Thus the maximum production of amylase was observed at optimized conditions, which can be employed in large scale.
Authors and Affiliations
J Mamatha , V Suresh, AB Vedamurthy , B Shilpi , SD Shruthi
Keywords
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