Production of Adventitious Root and Saponin of Talinum paniculatum (Jacq.) Gaertn. in Temporary Immersion Bioreactor
Journal Title: Scholars Academic Journal of Biosciences - Year 2014, Vol 2, Issue 4
Abstract
Abstract: This research is conducted to identify the effects of immersion length and interval on Talinum paniculatum (Jacq.) Gaertn adventitious roots’ biomass and saponin content in temporary immersion bioreactor. Immersion intervals used in this research are 3, 6, and 12 hours with immersion lengths 1, 3, 5, and 7 minutes. Adventitious roots were induced from leaf explants grown on MS medium added by IBA 2 mg/L. Two grams adventitious root are cultured in temporary immersion bioreactor with each treatment and kept for 28 days. Results of this research indicated different biomass and saponin content caused by each treatment. The best combination was found on immersion length of 5 minutes and 12 hours interval which resulting 3.67 g fresh weight, growth speed 0.027 g/day, spot area of saponin 12.56 mm2/0.01 g dry-weight, and spot area thickness scale 4+. Keywords: adventitious roots, saponin, temporary immersion bioreactor, Talinum paniculatum (Jacq.) Gaertn
Authors and Affiliations
Yosephine Sri Wulan Manuhara, Nike Oktavia Sri Saputri, Alfinda Novi Kristanti
Keywords
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