Production of Amylase by Arthrobacter kerguelensis VL-RK_09 Isolated from Mango Orchards
Journal Title: Biotechnology Journal International - Year 2015, Vol 8, Issue 4
Abstract
Objectives: To optimize the cultural parameters for improved production of amylase by Arthrobacter kerguelensis VL-RK_09 isolated from Mango orchards of Vissannapet, Krishna District, A.P., India. Methods: The strain A. kerguelensis was screened initially for amylase production on Inorganic salts starch agar medium (ISP-4). The enzyme assay was performed as per the procedure described by Bernfield (1955). One amylase unit equals to that amount of enzyme needed to release 1 mg of reducing sugar (maltose as standard) for 15 min at 37°C. Attempts were also made to optimize cultural parameters such as pH, temperature, carbon and nitrogen sources affecting the production of amylase by the strain. Results: Maximal yields of amylase were recorded after 4 days of incubation in Inorganic salts starch medium with initial pH 7.0 and temperature 35°C. ISP-4 broth amended with sorghum flour (2%) and yeast extract (0.5%) with initial pH 7.0 inoculated with Arthrobacter kerguelensis VL-RK_09 and incubated at 30°C for 96 h resulted in improved production of amylase from initial 4.0 U to 10.4 U/mL. Conclusion: This is the first report on the production and optimization of amylase by A. kerguelensis and further studies on purification and characterization of the enzyme are in progress.
Authors and Affiliations
Vijayalakshmi Muvva, Rajesh Kumar Munaganti, Krishna Naragani
Keywords
Related Articles
- EP ID EP237283
- DOI 10.9734/BBJ/2015/19383
- Views 109
- Downloads 0
Assessment of Methaemoglobin and Carboxyhaemoglobin Levels among Pregnant Women Infected with Hepatitis B Virus
Aims: The goal of this research is to determine plasma levels of MetHb and COHb in pregnant women with hepatitis B, which might enhance oxidative stress and hypoxemic condition of this state if it is not ameliorated on t...
Alkaline Cellulase Production by Penicillium mallochii LMB-HP37 Isolated from Soils of a Peruvian Rainforest
Alkaline cellulases are demanded by the textile industry for several purposes but commercial preparations showing activity at alkaline conditions are very scarce. Aim: To characterize a Penicillium strain isolated form s...
Effects of Initiating Antihypertensive Therapy with Amlodipine or Hydrochlorothiazide on Creatinine Clearance in Hypertensive Nigerians with Type 2 Diabetes Mellitus
Aims: To assess the effects of initiating antihypertensive therapy with amlodipine (AML) or hydrochlorothiazide (HCZ) for 48 weeks on creatinine clearance (Clcr) in hypertensive Nigerians with type 2 diabetes mellitus (D...
Thyroid Hormone Profile in Rwandan Students
Aim: To illustrate the thyroid hormone profile in young adult students at moderate altitude in Rwanda. Study Design: A population-based cross-sectional study. Place and Duration of Study: The study was conducted among he...
Influence of Type and Age of Primary Somatic Embryo on Secondary and Cyclic Somatic Embryogenesis of Cassava (Manihot esculenta Crantz)
This study investigated the influence of age of the cotyledons, cut from primary somatic embryos (PSE) developed from shoot meristems (SM) or immature leaf lobes (LL), on secondary somatic (SSE) and cyclic (CSE) embryoge...