Abstract

Objectives- Isolation of Cellulase enzyme producing bacteria from polluted water sample and optimization of growth parameters such as pH, Incubation temperature, incubation time, Concentration of Carboxy methyl cellulose for more enzyme production .Methods- Isolated bacteria were subjected to screen for Cellulase production on Carboxy methyl cellulose agar .The bacteria showing more clear zone around was selected and identified as Micrococcus roseus. The parameters used for the optimization of production were incubation temperature at 270C &370C , production medium pH range from 6 to 9 (i.e., 6, 7, 8 , 9), in 5 different concentration of Carboxy methyl cellulose (0.5, 1, 1.5, 2 & 2.5%), and incubation time (24, 48, 72hrs). Conclusion - Micrococcus roseus produce maximum cellulase at 1% of Carboxy methyl cellulose , 370C, pH 7 and at 48hrs. Bacterial cellulase production gain lesser importance especially Micrococcus sp, majority of works on cellulase production have been focused on fungi . This work is the easy and not an expensive method to produce cellulase and which has high demand in various industries and high cost in market

Authors and Affiliations