PROFILE OF GROWTH AND THE α-AMYLASE PRODUCTION FROM VARIOUS BACILLUS SPECIES
Journal Title: Innoriginal: International Journal Of Sciences - Year 2017, Vol 4, Issue 3
Abstract
Objective: In the present research work, three bacteria Bacillus subtilis, Bacillus licheniformis and Lactobacillus acidophilus were subjected to study their growth profile and alpha amylase production from them. Method: Nutrient agar and nutrient broth medium were used for their growth. In the nutrient broth, it was found that these bacteria were able to produce α-amylase. Production of α-amylase from these bacteria were studied through Iodine assay and DNSA. Results: It was investigated that DNSA technique is better than Iodine assay for the alpha amylase production from Bacillus species bacteria. Conclusion: Bacillus subtilis has better efficiency to produce alpha-amylase in nutrient medium, in compare to Bacillus licheniformis, Lactobacillus acidophilus
Authors and Affiliations
PRIYA VYAS, MRUNAL SHIRSAT
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