Progress of liquid chromatography and mass spectrometry-based single-cell proteomics
Journal Title: Biomedical Transformation - Year 2022, Vol 3, Issue 4
Abstract
Proteins are the main functional executor of cellular function. Due to the fact that proteins could not be amplified in vitro, the development of single-cell proteomics technology lags behind single-cell genomics and transcriptomics technologies. The traditional proteomics technology detects the average protein expression levels from large amounts of cells; nevertheless, it is unable to address the cell subtypes and heterogeneity among individual cells. Analysis of proteins at the single-cell level can help characterize the molecular basis of cell phenotypes and heterogeneity. With the rapid development of mass spectrometers, mass spectrometry-based methods have pushed single-cell proteomics to a new level. In this review, we summarized the recent research progress of single-cell proteomics methods based on LC-MS/MS, including single cell selection, sample preparation, isotope labelling technology, peptide separation, mass spectrometry analysis and data analysis, and its applications in biomedical research. The challenges and prospects of single-cell proteomics in the future are also prospected. Advances in single cell proteomics will provide new ideas and methods for biomedical research and deepen our understanding of human health and disease.
Authors and Affiliations
Huo Zian, Guo Yueshuai, Wang Yue, Situ Chenghao, Guo Xuejiang
Keywords
Related Articles
- EP ID EP766861
- DOI 10.12287/j.issn.2096-8965.20220411
- Views 13
- Downloads 0
The role of mast cells in pulmonary infectious diseases
Pulmonary mast cells are mainly distributed in the surrounding of pulmonary vessels, bronchi, and mucosa. When pathogens invade, they are directly phagocyted by mast cells through endocytosis. In addition, pulmonary mast...
Maternal obesity and oocyte quality
With an escalating global epidemic of obesity, the prevalence of obesity among reproductive women continues to rise. It has been widely reported that maternal obesity has profound effects on female reproduction. Of them,...
Establishment of data consortium for responding medical uncertainty during public health emergencies
The problems of medical decision-making caused by medical uncertainty during the coronavirus disease 2019 (COVID-19) pandemic were reviewed, and the potential mechanisms were analyzed. Medical uncertainty could lead to i...
Research progress on immunometabolism regulatory mechanisms of tuberculous granuloma
Tuberculosis (TB) remains a significant threat to human health. Tuberculous granuloma is a typical pathological feature of TB and also a "refuge" for Mycobacterium tuberculosis (Mtb) to escape host immune defense and ach...
Clinical application of biological graft vaginoplasty in patients with MRKH Syndrome
Objective This study aimed to evaluate the psychological health, quality of life, and sexual function in patients with Mayer-Rokitansky-Küster-Hauser (MRKH) syndrome treated with biological graft vaginoplasty. Methods Th...