Proliferation of Shoot from First Leaf of Carthamus tinctorius L. (Safflower)
Journal Title: SSR Institute of International Journal of Life Sciences (SSR-IIJLS) - Year 2018, Vol 4, Issue 3
Abstract
Callus induction and in vitro plantlet regeneration system for safflower (Carthamus tinctorius L.) using the first leaf were optimized by studying the influence on organogenesis of seedling age, media factor, growth regulator and excision orientation. Supplementation medium with auxin and cytokinin ratio >1 enhances the growth rate of callus culture. Growth regulators IAA, NAA, BAP, kinetin in the medium were found effective for callus induction and regeneration in all explants. The BAP 5mg/l, NAA 1mg/l, 5-7 explants and callus derived from cotyledonary. Explants were cut from basal region of cotyledon of 5–7 days old seedlings. As compared to the standard media SH-M and B-5 growth were superior in MS medium. Capitula induction was observed in callus mediated shoot from cotyledon and with sucrose, IAA, NAA and BAP. The well-developed plantlet was transferred to the field.
Authors and Affiliations
Sneha Mendhe, Sana Sheikh
Keywords
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- EP ID EP670900
- DOI 10.21276/ijlssr.2018.4.3.6
- Views 86
- Downloads 0
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