Properties of Aspergillus flavus Cellulase Produced from Solid State Fermentation of Brewers’ Spent Grain (BSG) as Substrate
Journal Title: Biotechnology Journal International - Year 2016, Vol 13, Issue 1
Abstract
The production of cellulase from Aspergillus flavus isolated from Brewers’ Spent Grain (BSG) undergoing bio-deterioration was carried out using solid state fermentation method. Out of a total of four fungal isolates with cellulase producing potentials, Aspergillus flavus with a clearance zone of 5.0cm was the best hyper-producer and was selected for optimization and cellulase production. Two different enzyme production media containing either yeast extract or potassium nitrate (KNO3) served as sources of nitrogen to the Aspergillus flavus. The crude activity of the potassium nitrate (KNO3) option (28,170 Units) was significantly higher than the crude cellulase from yeast extract option (18,600 Units). Ammonium sulphate precipitation at 60%, 80%, and 100% had cellulase activities 14600 units, 4400 unit, and 2800uits in yeast extract amended experimental option. In addition, ammonium sulphate precipitation at 60%, 80%, and 100% gave cellulase activities of 21130 units, 1700 units, and 1110 units, respectively in the KNO3 amended experimental option. Thus, 60% ammonium sulphate precipitation gave the best cellulase activity. The optimum pH of Aspergillus flavus cellulase was 6.0, while the optimum temperatures were between 50°C to 60°C. Tolerance to some alkali and alkaline–earth metals was also established, and probable co-factor behaviour of manganese ion to this cellulase was also established. Good government policies that support high import duties on foreign enzymes, emphasis on local production are advocated for in order to stamp out the economic leakages accruing through enzyme importation into Nigeria.
Authors and Affiliations
F. A. Orji, E. N. Dike, A. K. Lawal, Y. L. Suberu, B. Ita, G. N. Elemo
Keywords
Related Articles
- EP ID EP237866
- DOI 10.9734/BBJ/2016/24882
- Views 88
- Downloads 0
Enhanced Biodegradation of Spent Lubricating Oil Contaminated Soil Using Poultry Litter
Enhanced biodegradation of spent lubricating oil contaminated soil using (40% w/w) poultry litter was studied for a period of 56 days. The bacterial count ranged from 1.7×106 cfu/ml – 4.0×106 cfu/ml for oil free soil (OF...
Listeria spp. in Raw Cow and Goat Meat in Port Harcourt, Nigeria
Aims: To determine the occurrence of Listeria monocytogenes and other Listeria spp. in raw cow and goat meat. Study Design: This work was based on a completely randomized design with two replication and the average value...
Attempt to Establish Direct Gene Transformation System to Seeds of Sweet Potato (Ipomoea batatas) Using Electroporation Method
Aims: The purpose of this study is to get transformants by using the seeds of sweet potato and the instrument of NEPA21, an electroporation method. Study Design: As the first step, we used grafting method to get seeds fr...
Biodegradation of Indeno (1, 2, 3 - c, d) Pyrene and Dibenzo (A, H) Perylene by Aerobic Heterotrophic Bacteria and Cyanobacteria in Brackish Water
Biodegradation of indeno (1, 2, 3- c, d) pyrene and dibenzo (a, h) perylene; high molecular weight PAHs by isolates of aerobic heterotrophic bacteria, cyanobacteria and its consortium from Boodo Creek characterized with...
Effects of Length of Fermentation and Storage Media on Chemical Properties of Palm Oil
This study examined the effects of different length of fermentation and storage media on quality characteristics of palm oil produced at the processing centre of Department of Agricultural and Bio-Environmental Engineeri...