Properties of Aspergillus flavus Cellulase Produced from Solid State Fermentation of Brewers’ Spent Grain (BSG) as Substrate
Journal Title: Biotechnology Journal International - Year 2016, Vol 13, Issue 1
Abstract
The production of cellulase from Aspergillus flavus isolated from Brewers’ Spent Grain (BSG) undergoing bio-deterioration was carried out using solid state fermentation method. Out of a total of four fungal isolates with cellulase producing potentials, Aspergillus flavus with a clearance zone of 5.0cm was the best hyper-producer and was selected for optimization and cellulase production. Two different enzyme production media containing either yeast extract or potassium nitrate (KNO3) served as sources of nitrogen to the Aspergillus flavus. The crude activity of the potassium nitrate (KNO3) option (28,170 Units) was significantly higher than the crude cellulase from yeast extract option (18,600 Units). Ammonium sulphate precipitation at 60%, 80%, and 100% had cellulase activities 14600 units, 4400 unit, and 2800uits in yeast extract amended experimental option. In addition, ammonium sulphate precipitation at 60%, 80%, and 100% gave cellulase activities of 21130 units, 1700 units, and 1110 units, respectively in the KNO3 amended experimental option. Thus, 60% ammonium sulphate precipitation gave the best cellulase activity. The optimum pH of Aspergillus flavus cellulase was 6.0, while the optimum temperatures were between 50°C to 60°C. Tolerance to some alkali and alkaline–earth metals was also established, and probable co-factor behaviour of manganese ion to this cellulase was also established. Good government policies that support high import duties on foreign enzymes, emphasis on local production are advocated for in order to stamp out the economic leakages accruing through enzyme importation into Nigeria.
Authors and Affiliations
F. A. Orji, E. N. Dike, A. K. Lawal, Y. L. Suberu, B. Ita, G. N. Elemo
Keywords
Related Articles
- EP ID EP237866
- DOI 10.9734/BBJ/2016/24882
- Views 110
- Downloads 0
Potential of Azadirachtin and Neem (Azadirachta indica) Based Saponins as Biopesticides for In vitro Insect Pests Cellulase (Beta-1,4-Endoglucanase) Enzyme Inhibition and In vivo Repellency on Tribolium castaneum
Aims: The work was undertaken to identify the role of Neem derived compounds (saponins and azadirachtin) on the digestive cellulose hydrolyzing enzyme activity of red flour beetle (T. castaneum), rice grasshopper (Oxya...
Optimization of Polyphenols Extraction Method from Kola Nuts (Cola nitida Vent. Schott & Endl.) Using Experimental Design
Aims: The aim of this study is to optimize extraction process of phenolics compounds from kola nuts by using experimental design. Study Design: Kola nuts were collected in October 2014-February 2015 in south of Côte d’Iv...
Molecular Identification of Biodiversity of Fusarium species Isolated from Wilted Oil Palm and Date Palm in Nigeria
Biodiversity of sixteen Fusarium isolates, isolated from the roots of oil palm and date palm in Nigeria was studied. Ten Fusarium isolates out of the sixteen were obtained from the oil palm while six isolates were from d...
Ethnobotanical, Nutritional, Phytochemical and Antimicrobial Studies of Garcinia xanthochymus Fruit Extracts
Aim: An attempt has been made to document the ethnobotanical values of the fruits of G. xanthochymus in and around the Similipal Biosphere Reserve (SBR) forest and evaluate the nutritional, phytoc...
Towards Efficient In vitro Regeneration of Cowpea (Vigna unguiculata L. Walp): A Review
Cowpea is a crop of tremendous economic and ecological values particularly in sub-Saharan Africa, where over 80% of the crop is produced and consumed. Due to heavy attack by pests and diseases, actual yield does not exce...