Towards Efficient In vitro Regeneration of Cowpea (Vigna unguiculata L. Walp): A Review
Journal Title: Biotechnology Journal International - Year 2015, Vol 7, Issue 4
Abstract
Cowpea is a crop of tremendous economic and ecological values particularly in sub-Saharan Africa, where over 80% of the crop is produced and consumed. Due to heavy attack by pests and diseases, actual yield does not exceed 20% of the crop’s potential in most of the production regions. Shortages of genes to combat biotic stresses in the germplasm and sexual incompatibility with wild relatives are major impediments in cowpea improvement. Genetic modification of cowpea with relevant genes can address these problems. Establishment of reproducible regeneration system is a prerequisite for genetic transformation of cowpea using transgenic technology. Cowpea is among the most recalcitrant crops for manipulation under In vitro condition especially via de novo process. However, strategies to regenerate cowpea under In vitro conditions have evolved steadily in the last three decades. In this review, we give a summary of cowpea regeneration work carried out so far and discussed approaches employed as well as challenges of developing efficient regeneration systems in cowpea.
Authors and Affiliations
Lawan Abdu Sani, Inuwa Shehu Usman, Muhammad Ishiaku Faguji, Sunusi Muhammad Bugaje
Biodiesel Fuel Production from Palm, Sunflower Waste Cooking Oil and Fish Byproduct Waste as Renewable Energy and Environmental Recycling Process
Significant of the Study: Biomass is renewable, organic, plant and animal derived source of biomaterial that can be converted into different forms of biofuel, bioplastic, bio-solvent, and bioenergy using different biotec...
Antifungal Activity of Camel Faeces with Special Reference to Dermatophytes
Aim: The present study was conducted to evaluate the antifungal activity of camel faeces on some pathogenic fungi. Study Design: This is a descriptive evaluation study. Methodology: Camel faeces was extracted following H...
Phenotypic and Genotypic Characterization of Phosphate Solubilizing Bacteria Isolated from the Errachidia Province, Morocco
Aims: The aim of this study was to characterize phenotypically and genotypically bacterial isolated from the soils of the Errachidia province in order to select the ones having the potential to solubilize the inorganic p...
Prospecting Filamentous Fungi for Amylase Production: Standardization of Aspergillus japonicus Culture Conditions
Aims: Prospecting different filamentous fungi for high production of amylases in standard conditions for future application in biotechnology industries. Methodology: Samples were collected in different field areas in the...
Isolation and Identification of Microorganisms from Crude oil Contaminated Soils of Dar es Salaam, Tanzania
Aims: The aim of this study was to isolate and identify microorganisms in crude oil polluted soils in Eastern Tanzania. Study Design: Isolation and identification of microorganisms using conventional and molecular techni...