Relationship of Genomic G+C Content between Phages/Plasmids and Their Hosts
Journal Title: Biotechnology Journal International - Year 2015, Vol 9, Issue 1
Abstract
Aims: We compared genomic G+C content between bacteriophages/plasmids and their host genomes of 46 species to reveal their relation. To examine the large deviation in the G+C content between bacteriophages and their host genomes, the ancestral bacteriophage which infected early was estimated using homologous genes of bacteriophages based on G+C content at the third codon positions. Place and Duration of Study: Department of Clinical Laboratory Science, Graduate Course of Medical Science and Technology, School of Health Sciences, Kanazawa University, Japan. Methodology: The bacteria employed in this study consist of 6 species from Archaea and 40 species from Eubacteria. Orthologs were identified by the two-directional best hit approach of homology search. A phylogenetic tree was obtained by multiple sequence alignment of homologous genes. The ancestral bacteriophage which infected early was estimated based on G+C content at the third codon positions. We assumed that the two bacteriophages have evolved from a common ancestor, and their identical codons were thought to represent their ancestor type. Results: The relationship of G+C content between bacteriophages/plasmids and host genomes was almost linear. Three bacteriophages were largely deviated from the linear relation. A phylogenetic tree obtained using the orthologs of Mycobacterium smegmatis indicated which bacteriophage branched early. Assuming that the G+C content of identical codons represents their common ancestor, the ancestor was estimated that it had similar G+C content with its host.
Authors and Affiliations
Hiroshi Nakashima, Keiko Homma, Kazuhiro Mawatari
Keywords
Related Articles
- EP ID EP237311
- DOI 10.9734/BBJ/2015/20046
- Views 116
- Downloads 0
In vitro Pepper (Capsicum annuum L.) Anther Culture: Can be Affected Via Vitamins B?
Pepper (Capsicum annuum L.) is a vegetable in family Solanaceae and has a very important place and development potential in the field of plant breeding and biotechnology. It is at the forefront of haploid plant acquisiti...
Screen House and Field Resistance of Taro Cultivars to Taro Leaf Blight Disease (Phytophtora colocasiae)
Introduction: Taro leaf blight disease cause by Phytophtora colocasiae has become an economic disease in Cocoyam growing regions of Cameroon. Aims: To screen for resistance 10 improved and 4 local cultivars of taro again...
Investigation the Consumption of Aromatic Hydrocarbons by Anoxybacillus rupeinsis Ir3 (JQ912241) using FTIR and HPLC Analyses
Aims: To confirm the ability of Anoxybacillus rupiensis strain Ir3 (JQ912241) to utilize the aromatic compounds using FTIR and HPLC analyses. Study Design: Experimental study. Place and Duration of Study: Department of...
Integration of Reticuloendotheliosis Virus in Most of Tanzanian Fowl Pox Virus Isolates is not attributed to Imported Commercial Fowl Pox Vaccines
Aim: To investigate integration of reticuloendotheliosis virus (REV) in the Tanzanian fowlpox virus (FWPV) field isolates, and the imported commercial fowl pox vaccines currently used in the country. Study Design: Exper...
Charge Transference Detection through Cyclic Voltammograms in the Interaction of Cratylia mollis Seed Lectin-carbohydrate Inhibitor
Aims: To evaluate the charge transference and the electrochemical potential of Cratylia mollis seed lectin, Cramoll 1,4, adsorbed on Nafion beads after the interaction of glucose ligand. Study Design: Based on detection...