Relationship of Genomic G+C Content between Phages/Plasmids and Their Hosts
Journal Title: Biotechnology Journal International - Year 2015, Vol 9, Issue 1
Abstract
Aims: We compared genomic G+C content between bacteriophages/plasmids and their host genomes of 46 species to reveal their relation. To examine the large deviation in the G+C content between bacteriophages and their host genomes, the ancestral bacteriophage which infected early was estimated using homologous genes of bacteriophages based on G+C content at the third codon positions. Place and Duration of Study: Department of Clinical Laboratory Science, Graduate Course of Medical Science and Technology, School of Health Sciences, Kanazawa University, Japan. Methodology: The bacteria employed in this study consist of 6 species from Archaea and 40 species from Eubacteria. Orthologs were identified by the two-directional best hit approach of homology search. A phylogenetic tree was obtained by multiple sequence alignment of homologous genes. The ancestral bacteriophage which infected early was estimated based on G+C content at the third codon positions. We assumed that the two bacteriophages have evolved from a common ancestor, and their identical codons were thought to represent their ancestor type. Results: The relationship of G+C content between bacteriophages/plasmids and host genomes was almost linear. Three bacteriophages were largely deviated from the linear relation. A phylogenetic tree obtained using the orthologs of Mycobacterium smegmatis indicated which bacteriophage branched early. Assuming that the G+C content of identical codons represents their common ancestor, the ancestor was estimated that it had similar G+C content with its host.
Authors and Affiliations
Hiroshi Nakashima, Keiko Homma, Kazuhiro Mawatari
Keywords
Related Articles
- EP ID EP237311
- DOI 10.9734/BBJ/2015/20046
- Views 119
- Downloads 0
Gamma Radiation: Physical Elicitor for In vitro Culture Techniques of Cajanus cajan
Gamma rays are very important in mutation breeding and in in vitro mutagenesis in order to develop required features of plants and increase the genetic variability. Cajanus cajan when subjected to absorbed doses 30 Gy, 5...
Molecular Relatedness and Morpho-Agronomic Characteristics of Congolese Accessions of Cassava (Manihot esculenta Crantz) for Breeding Purposes
Cassava serves as primary staple food of millions of people in the tropics and subtropics, and is used as a carbohydrate source in animal feed. Knowledge of agro-morphological characteristics and genetic relatedness is e...
Effects of Input Variables on the Conversion of 5 Ton/h Processed Tomato Juice in a Triple-Effect Evaporator
Tomato is grown in many regions of the world; and processed tomato products account for 80% of total tomato consumption. A worldwide tomato processing industry has since evolved with a range of technologies which are abl...
Phytochemical and Antimicrobial Screening of Root Extracts of Dacryodes edulis
Aims: To screen extracts of Dacryodes edulis phytochemically. To assay the said extracts for antimicrobial activity. To validate or otherwise claims by traditional medicine practitioners on Dacryodes edulis. Place and Du...
Survey and Indexing of Weeds Growing around Potato Fields for Their Role as an Inoculum Source for Potato leafroll virus (PLRV)
A survey and indexing studies was carried out on weeds plants growing around potato fields to identify their role as an inoculum source for Potato leafroll virus (PLRV). Symptomatic and asymptomatic foliage samples of 26...