Protective eff ect of rosemary (Rosmarinus offi cinalis) extract on naphthalene induced nephrotoxicity in adult male albino rat
Journal Title: Journal of Interdisciplinary Histopathology - Year 2015, Vol 3, Issue 1
Abstract
Background: Naphthalene (NA) is a common environmental contaminant and is abundant in tobacco smoke. Rosemary (Rosmarinus officinalis) is a herb commonly used as a spice and flavoring agents in food processing and is useful in the treatment of many diseases. Aim of the work: To study the nephrotoxicity of NA and to evaluate the possible protective role of rosemary extract in adult male albino rat. Materials and Methods: 25 animals were divided into three groups: Group I (Control group), Group II (NA treated group) received NA at a dose of 200 mg/kg/day dissolved in 5 ml/kg corn oil orally by gastric tube, Group III (protected group) received rosemary extract (10 ml/kg/day) followed after 60 min by NA at the same previous dose orally by gastric tube. The experiment lasted 30 days. The following parameters were studied: Biochemical assessment of renal function, histological, immunohistochemical, morphometric studies and statistical analysis of the results. Results: NA treatment resulted in a highly significant increase in the mean values of serum urea and creatinine. NA induced histological changes in the form of glomerular congestion. Some glomeruli demonstrated marked mesangial expansion and hence that Bowman’s spaces were almost completely obliterated. Shrinkage of renal glomeruli with widening of Bowman’s spaces could also be seen. Focal tubular dilatation with appearance of casts inside the tubules was observed. Congested peritubular blood vessels and interstitial hemorrhage were also seen. The medullary region demonstrated vascular congestion and fibrosis. Focal cellular infiltration was presented in the interstitium. The renal cortex of NA treated rats showed a noticeable down regulation in alkaline phosphatase positive immunoreactive cells in some proximal convoluted tubules. NA induced up regulation of positive immunoreaction for inducible nitric oxide synthase in the proximal and distal convoluted tubules as well as in the collecting tubules. All previous results were significantly reversed in the protected group compared with NA treated group. Conclusions: It is concluded that NA has a harmful effect on rat kidney. Rosemary extract had a protective role against renal injury induced by NA and this effect may be attributed to its antioxidant and anti-inflammatory activity.
Authors and Affiliations
Neveen El-sherif, Noha Issa
Keywords
Related Articles
- EP ID EP163395
- DOI 10.5455/jihp.20150213020636
- Views 110
- Downloads 0
Cytomorphometric Analysis of Oral Premalignant and Malignant Lesions Using Feulgen Stain and Exfoliative Brush Cytology
Objective: Oral squamous cell carcinoma (OSCC) is the sixth most common cancer worldwide and accounting for 90% of cancers of oral cavity. Tobacco abuse has been proved to be the major risk factor in the development of O...
A rare fi nding of coexisting fi broadenoma and duct ectasia in axillary ectopic breast tissue
Ectopic breast tissue (EBT) can present anywhere along the milk line from axilla to groin. It can harbor all the pathological conditions as that of the normal breast though incidence is very low. Fibroadenoma is rare in...
Paratesticular liposarcoma of the spermatic cord: A rare case
Spermatic cord liposarcomas (LPS) are rare representing 7% of paratesticular sarcomas. The most present as slow growing inguinal or scrotal masses that need to be differentiated from inguinal hernias, testicular tumors,...
Anabolic Androgen-induced Intrahepatic Cholestasis Presented With Normal γ-Glutamyl-Transpeptidase
The indisputable hepatotoxicity of anabolic androgen steroids has already been well substantiated in the literature, eventhough the exact pathophysiologic mechanisms are still being elucidated. Androgen-induced liver inj...
Primary mesenteric carcinoid tumor: A rare entity
Mesenteric tumors are uncommon lesions. Primary mesenteric carcinoid is a rare entity with only a few reported cases. Therefore, a thorough examination of the resected bowel is necessary to rule out the presence of possi...