Protective Effect of Alchornea laxiflora Methanolic Leaf Extract on CCL4-induced Hepatotoxicity and Reproductive Toxicity in Male Wistar Rats
Journal Title: Journal of Pharmaceutical Research International - Year 2016, Vol 14, Issue 1
Abstract
Background: Alchornea laxiflora is a medicinal plant with immense health benefits. Aim: To verify the protective effects of methanol extract of Alchornea laxiflora (MEAL) leaf on hepatotoxicity and reproductive toxicity induced by CCl4. Settings and Design: Extraction and administration of Bioactive extract. Place and Duration of Study: Department of Biochemistry, University of Medical Sciences, Ondo City, Ondo State, P.M.B 536. Between November 2015 - July 2016. Materials and Methods: Thirty five Albino Wistar rats were randomized into seven groups of five animals each, group 1 animals which served as positive control, received normal saline, group 2 animals which served as negative control, received 1 ml/kg body weight of CCl4 only for a day while groups 3-7 received 0.1, 0.5, 1.0,10.0 and 50 mg/kg body weight of methanol extract Alchornea laxiflora (MEAL) leaf respectively for seven days after receiving a single dosage CCl4 (1 ml/kg body weight) for a day. Activities of Gamma glutamyl transferase (GGT), Glutathione-S-transferase (GST), Alanine aminotransferase (ALT) and Alkaline phosphatase (ALP) were assayed to verify the effects of the plant extract on the liver while percentage sperm motility and sperm morphology was assayed to check the effects of the plant extract on reproductive system/organs. Statistical Analysis: The data were analysed by SPSS (version 2.1). Result and Conclusion: Graded dosage (0.1-50 mg/kg bodyweight) of methanol extract of Alchornea laxiflora leaf significantly (p≤0.05) reversed the toxic effects of CCl4 on the liver and reproductive organs of wistar rats in a dose dependent manner as compared to group 2 animals. This may be due to the secondary metabolites content of Alchornea laxiflora, which have been shown to have profound antioxidant potentials. It could be deduced that Alchornea laxiflora leaf extract can reverse hepatotoxicity and reproductive toxicity and by extension cure infertility in rats.
Authors and Affiliations
E. S. Uhunmwangho, N. O. Rasaq, O. Ojemekele
Keywords
Related Articles
- EP ID EP342100
- DOI 10.9734/BJPR/2016/29807
- Views 76
- Downloads 0
Solubility Enhancement of a BCS Class II Drug Using Granulated Fumed Silica as an Adsorbent
Aim: The aim of this study was to improve solubility and dissolution characteristics of Ibuprofen using granulated fumed silica (Aeroperl®300) as a carrier. Methods: Ibuprofen-silica complex was prepared by solvent evap...
A New Approach for the Treatment of Diabetes and Nicotine Induced Cataract by Modulating Specific Ion-Channels Function in Rat Lens
Aim: Cataract is an eye disease characterized by a cloudiness of the normally transparent crystalline lens. Diabetes and smoking are the known risk factors for cataract development. It is well known fact that inorganic i...
Effect of Cymbopogon citratus Decoctions on Gasoline Vapour-induced Reproductive Toxicity in Female Rats
Aims: Recent research indicates that plant molecules, particularly those that are rich with antioxidant, anti-inflammatory and immune modulatory constituents, can modify and prevent the detrimental effects of gasoline co...
A QSAR Study of New Caffeine Derivatives with Epithelial Anticancer Activity
Aims: To study and propose new caffeine derivatives with epithelial anticancer activity using quantum chemistry methods and multivariate analysis (PCA, HCA, PLS and PCR). Place and Duration of Study: Laboratory of Modeli...
Archachatina marginata Haemolymph Potentiates Hypoglycemic Effect by Mimicking Insulin in Streptozotocin-Induced Diabetic Rat
Aims: This study investigates the hypoglycemic potential of Archachatina marginata haemolymph and its mechanism in streptozotocin -induced diabetic rats. Study Design: One factor experimental design. Place and Duration o...