PROTECTIVE EFFECTS OF METFORMIN AGAINST HEPATORENAL INJURY IN HIGH-FAT DIET/STREPTOZOTOCINE-INDUCED DIABETIC MICE
Journal Title: Kocatepe Medical Journal - Year 2022, Vol 23, Issue 3
Abstract
OBJECTIVE: In this study, it was aimed to investigate the antioxidant effect of metformin treatment on liver and kidney tissues in mice with experimental type 2 diabetes induced by a high-fat diet and streptozotocin. MATERIAL AND METHODS: Twenty-one adult C57BL6 male mice were randomly divided into three groups as Control group (C), Diabetes group (D), and Diabetes + Metformin group (D + MTF). Groups D and D + MTF were fed a high-fat diet with 60% fat for four weeks. After the 4th week, 30 mg/kg streptozotocin was applied intraperitoneally, and type 2 diabetes was induced. 300 mg/kg/day Metformin was given by gavage. Glutathione (GSH), catalase (CAT) and malondialdehyde (MDA) levels in liver and kidney samples were measured by spectrophotometric method. Tissue sections were stained with Hematoxylin and Eosin and evaluated histomorphologically. RESULTS: The results showed that MDA levels increased in the liver and kidney tissues of diabetic animals. It was found that MTF treatment decreased MDA levels in both tissues and increased GSH and CAT levels, but this increase was not significant in the liver (p> 0.05), while it was significant in the kidney (p <0.05). In addition, in the histopathological evaluation results, vacuolization, occasional necrosis and synozoid occlusion were observed in rare areas in the D+MTF group, while intense vacuolization, high rates of synozoidal occlusion and necrosis were observed in group D livers. In kidney tissue, the tubular structure in the group C was normal while the tubules were enlarged in group D, and there were dense bleeding areas. In the D+MTF group, vacuoles were observed in the tubule structures in rare areas. CONCLUSIONS: The results of this study show that oxidative stress occurs in tissues in diabetes and that metformin treatment prevents tissue damage by showing a corrective effect on the increased oxidative stress in diabetes.
Authors and Affiliations
Songül DOĞANAY, Özcan BUDAK, Nurten BAHTİYAR, Veysel TOPRAK
Keywords
Related Articles
- EP ID EP706858
- DOI https://doi.org/10.18229/kocatepetip.840797
- Views 68
- Downloads 0
USE OF COLCHICINE FOR POST PERICARDIECTOMY SYNDROME IN PATIENTS WITH CORONARY ARTERY BYPASS GRAFT AND ITS EFFECTS ON SURGICAL INTERVENTION
OBJECTIVE: Postpericardiotomy syndrome (PPS) continues to be one of the most common causes of morbidity after cardiac surgery despite all developments. We investigated whether colchicine is more effective than expected i...
COMPARISON OF SCAN LENGTH AND RADIATION DOSE PARAMETERS IN CONSECUTIVE THORACIC AND ABDOMINAL CT EXAMINATIONS
OBJECTIVE: The aim of this study is to evaluate the scan length variability and the effect of the scan length on radiation dose parameters in consecutive thoracic and abdominal CT examinations. MATERIAL AND METHODS: P...
A RARE DIAGNOSIS IN CHILDREN: JUVENILE FIBROMYALGIA
Juvenile fibromyalgia (JFM) is a chronic pain condition in children. This extremely rare disease was first described in 1985. Although it is seen at young ages, its incidence is increasing in adolescents. Girls are more...
DOĞUMDA KORDON KANINDA KURŞUN VE KADMİYUM DÜZEYLERİ
OBJECTIVE: Lead and cadmium exposure during pregnancy has negative effects on mother and baby. Karabuk province is the center of the iron and steel industry and is a risky region in terms of lead and cadmium exposure. Th...
CERVICAL DYSPLASIA RISK IN MULTIPLE SCLEROSIS PATIENTS
OBJECTIVE: In this study, we aimed to investigate the relationship between Multiple Sclerosis (MS) disease and the development of cervical dysplasia. MATERIAL AND METHODS: This is a retrospective case-control study that...