Protein-Protein Interaction Between the Short and Long Form of Grg Family of Co-Repressors
Journal Title: International journal of Emerging Trends in Science and Technology - Year 2014, Vol 1, Issue 7
Abstract
The Grg family of corepressor proteins lacks DNA-binding domain and these are recruited to the promoter region by interacting with DNA-binding transcription factors. Recruitment to the promoter by DNA-binding transcriptional factors results in transcriptional repression. There are five member of Grg proteins namely Grg1-5. Grg1-4 are the long forms and Grg5 is the short form of Grg family of corepressor. It has been reported that Grg proteins make tetramers to mediate the repression. In a collocatiozation assay, the full length myc-mGrg3 was transfected in COS7 cells along with mGrg5 and mGrg1∆280 fused with GFP. The colocalization assay indicates that full length myc-mGrg3 interacts with short form mGrg5-GFP by changing the localization pattern of mGrg5-GFP from cytoplasm to nucleus. In addition, we have also shown that Grg proteins interact with each other via N-terminal end, since myc-mGrg3 could not alter the translocation of non nuclear truncated form mGrg1∆280-GFP from cytoplasm into the nucleus. The inability of myc-mGrg3 to translocate mGrg1∆280-GFP indicates that Grg protein interact with each other only via N-terminal end. In conclusion, results we show here suggest that Grg protein interact with each other via N-terminal end and that this interaction alters the localization pattern of interacting Grg proteins.
Authors and Affiliations
Zulfiqar Ali Laghari
Keywords
Related Articles
- EP ID EP220985
- DOI -
- Views 118
- Downloads 0
Web Based Security using oPass System: A Survey
Password authentication is an essential form of user authentication both on the Internet and for internal organizational computing systems. Password protection schemes are used to protect relatively low-sensitivity syste...
A DSTATCOM Topology with Fast-Acting DC-Link Voltage Controller to Compensate AC and DC Loads
The transient response of the distribution static com- pensator (DSTATCOM) is very important while compensating rapidly varying unbalanced and nonlinear loads. Any change in the load affects the dc-link voltage directly....
Electromagnetic Field Exposure – A Health Hazard
The world around us produces electromagnetic fields. However, these are generally of low intensity. We cannot see or hear these radiations. Technological products generate intense electromagnetic fields. Bioelectrical si...
Collaborative Location Privacy through Buffering
Location based services available in the smart phones are used to gain information about the surroundings. Users query the location based server by passing their position information thereby losing their privacy. A user...
Similar Characteristics of Fibrillar form of β-Amyloid Peptide Fractions from Mice Brain affected by Systemic Amyloidosis
Enhanced expression of amyloid β-peptide (Aβ) and deposition is the main causative factor in Alzheimer’s disease (AD). Factors that lead to the genesis of accumulation and toxicity of Aβs are yet to be identified. While...