Prothrombogenic thrombocytic phenotype in patients with nonproliferative diabetic retinopathy
Journal Title: Міжнародний ендокринологічний журнал - Year 2018, Vol 14, Issue 2
Abstract
Background. Patients with type 2 diabetes mellitus (DM) have increased reactivity of thrombocytes (Tc), the causes of which remain unclear. The hypothesis of the dependence of Tc receptors functional activity on the individual reactivity of the organism is tested. The purpose of the study was to establish whether the individual reactivity of Tc differs in patients with diabetic nonproliferative retinopathy (DNPR). Materials and methods. The study included 19 patients (19 eyes) with type 2 DM, who, according to the results of the clinical and instrumental examination and according to the Early Treatment of Diabetic Retinopathy Study classification, had mild DNPR. Ophthalmological examination was performed before the beginning of treatment and included the collection of anamnesis, visual acuity study with optimal optical correction, tonometry, gonioscopy, biomicroscopy, ophthalmoscopy, optical coherence tomography (Stratus OCT apparatus). Thrombocytes were isolated by centrifugation from citrated peripheral blood of patients. To activate Tc, agonists were used: adenosine diphosphate (2.5 μM), adrenaline (2.5 μM), angiotensin II (1.0 μM), platelet activating factor (75.0 μM) and collagen (1.0 mg/ml). The Tc aggregation was evaluated by a spectrophotometric method on the ChronoLog aggregometer (USA). Results. The revealed individual reactivity of Tc in patients with DNPR was manifested by the features of thrombogenesis induction. A hyperreactivity of Tc to three agonists was detected: adrenaline, collagen and angiotensin II, which was characteristic of prothrombogenic phenotype. Depending on the functional activity of the investigated receptors, two main clusters of Tc receptors were identified, which were equal by the activity of α2-adrenoreceptors and angiotensin type 1 receptors, but differed by the response of Tc to collagen. Conclusions. Determination of the prothrombogenic phenotype and clusters of functionally active Tc receptors opens the possibility for evaluating the proaggregant effect of etiological and pathogenetic factors of type 2 DM in order to establish the factors of diabetic retinopathy progression.
Authors and Affiliations
A. S. Gudz, M. L. Maksimtsiv, S. V. Ziablitsev, S. Yu. Mogilevsky
Keywords
Related Articles
- EP ID EP299478
- DOI 10.22141/2224-0721.14.2.2018.130558
- Views 69
- Downloads 0
Comparison of Infertility Causes Structure in Men and Women in Tashkent and Andijan Regions of Republic of Uzbekistan According to Screening Data
The structure of the causes of infertility has been studied in 100 couples (200 people) with infertility in Tashkent and Tashkent region (50 families in each). It is found that among surveyed women in the genesis of infe...
Chornobyl Accident and Iodine Deficiency as Risk Factors of Thyroid Pathology in Population of the Affected Regions of Ukraine
The result of the Chornobyl nuclear power plant accident was the negative effect of radiation on the health of population of large areas of Ukraine and surrounding countries, the impact of this action will be shown over...
Activation of intracellular enzyme systems under the influence of pathogenetic factors of oncogenesis in patients with type 2 diabetes mellitus
Background. It is proved that patients with diabetes mellitus (DM) have an increased risk of cancer. The main factors of oncogenesis in DM are obesity, cytokine imbalance, hyperinsulinemia (HI), hyperglycemia. These diso...
Effect of Acute Power Load on the Parameters of Hormonal Response in Untrained Young Men During Weight Training Exercises
The article displays the results of experimental studies on the characteristics of the changes in steroid hormones cortisol and testosterone levels in the blood of untrained young men in response to the acute power load...
Use of Zinkit in Combination Treatment of Patients with Dwarfism
The article deals with the results of study of zinc content in children and adolescents with dwarfism due to somatotropic deficiency, and rationale for use of zinс-containing preparations (Zinkit, «Wörwag Pharma», German...