Proximate composition, phytochemical screening, GC-MS studies of biologically active cannabinoids and antimicrobial activities of Cannabis indica

Journal Title: Asian Pacific Journal of Tropical Disease - Year 2015, Vol 5, Issue 11

Abstract

Objective: To investigate the proximate composition, minerals analysis, phytochemical screening, gas chromatography-mass spectrometry (GC-MS) studies of active cannabinoids and antimicrobial activities of Cannabis indica (C. indica) leaves, stems, and seeds. Methods: Standard qualitative protocols of phytochemical screening were accomplished for the identification of biologically active phytochemicals. Minerals in plant samples were analyzed by using atomic absorption spectrophotometer. The resins of C. indica were analyzed for medicinally active cannabinoid compounds by GC-MS. The sample for GC-MS study was mixed with small quantity of n-hexane and 30 mL of acetonitrile solution for the identification of cannabinoids. Agar well diffusion method was used for antibacterial activity. For antifungal activity, the tested fungal strains were sub-cultured on Sabouraud’s dextrose agar at 28 °C. Results: Mineral analysis revealed the presence of sodium, potassium, magnesium and some other minerals in all parts of C. indica. Phytochemical investigation showed the presence of alkaloids, saponins, tannins, flavonoids, sterols and terpenoids. C. indica divulged wide spectrum of antibacterial activities against Staphylococcus aureus, Bacillus cereus, Klebsiella pneumoniae, and Proteus mirabilis. The extracts of plant leaves, seeds and stems showed significant antifungal activities against Aspergillus niger, Aspergillus parasiticus, and Aspergillus oryzae. The biologically active cannabinoids of delta-9-tetrahydrocannabinol (25.040%) and cannabidiol (resorcinol, 2-p-mentha-1,8-dien-4-yl-5-pentyl) (50.077%) were found in Cannabis resin in high percentage. Conclusions: The findings of the study suggested that the existence of biologically active remedial cannabinoids in elevated concentrations and antimicrobial bioassays of C. indica make it a treasured source to be used in herbal preparation for various ailments.

Authors and Affiliations

Keywords

Related Articles

Micro-emulsion based emulgel: a novel topical drug delivery system

Topical delivery systems for drugs make localized administration of the drug anywhere in the body through ophthalmic, vaginal, skin and rectal routes. Topical formulations encompass a wide variety of formulations intende...

Is vitamin D defiiency associated with using veil in female garment workers?

Objective: To find out the determinant factors which are associated with the serum vitamin D status in the female garment workers. Methods: This study was a cross sectional design with a total of 154 childbearing female...

TLC profiles and antibacterial activity of Glinus oppositifolius L. Aug. DC. (Molluginaceae) leaf and stem extracts against bacterial pathogens

Objective: To determine the antibacterial activities and the thin-layer chromatography (TLC) fingerprint profiles of leaf and stem extracts of Glinus oppositifolius L. Aug. DC (G. oppositifolius). Methods: The leaves and...

Utilization of DVT Prophylaxis in non ICU Hospitalized Patients

The aim of this study was to assess all aspects of the routine clinical practices of DVT prophylaxis followed in the non ICU hospitalised (both medical & surgical) patients in various ward of multispecialty, tertiary car...

Magnitude of hepatitis B infection in pregnant women and in clinically suspected infectious hepatitis at a tertiary care hospital in Ghaziabad

Objective: To determine the magnitude of hepatitis B virus infection in pregnant women and in clinically suspected infectious hepatitis at a tertiary care hospital in Ghaziabad. Methods: This prospective study was conduc...

Download PDF file
  • EP ID EP228417
  • DOI 10.1016/S2222-1808(15)60953-7
  • Views 105
  • Downloads 0

How To Cite

(2015). Proximate composition, phytochemical screening, GC-MS studies of biologically active cannabinoids and antimicrobial activities of Cannabis indica. Asian Pacific Journal of Tropical Disease, 5(11), 897-902. https://europub.co.uk/articles/-A-228417