Purification and Characterization of A Novel CGTase from Alkaliphilic Bacillus species isolated from Lonar lake , India
Journal Title: International Journal of Life Science - Year 2018, Vol 6, Issue 2
Abstract
Production and purification of Cyclodextrin glycosyltransferase (CGTase) from Alkaliphilic Bacillus species isolated from Lonar lake , India was investigated in present study. Production was carried out using medium containing starch, yeast extract, peptone MgSO4.7H2O. the crude enzyme was collected by centrifugation and partially purified using ammonium sulphate precipitation method. This partially purified enzyme was further purified using phenyl sepharose column chromatography. The enzyme obtained had molecular weight of 79.20 kDa which is confirmed by SDS PAGE and Mass spectroscopy.
Authors and Affiliations
VA Shinde, RB Patil, PV Pawar
Keywords
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