Qualitative and quantitative determination of protopine in Fumaria spp. by TLC-densitometry method
Journal Title: Journal of BioScience and Biotechnology - Year 2012, Vol 1, Issue 3
Abstract
A rapid and accurate TLC-densitometry method for qualitative and quantitative determination of protopine has been developed. The best separation was achieved using a mobile phase chloroform – ethyl acetate – methanol – ammonium hydroxide (80:80:40:0.05, v/v/v/v). The results obtained by this method (CV% 3.4) corresponded well with those obtained by using a HPLC method. The reliability of the proposed method was proved through reproducibility test with alkaloid extracts from Fumaria spp.
Authors and Affiliations
Radka Vrancheva, Ivan Ivanov, Andrey Marchev, Atanas Pavlov
Keywords
Related Articles
- EP ID EP87218
- DOI -
- Views 103
- Downloads 0
Horse chestnut (Aesculus hippocastanum L.) as a biomonitor of air pollution in the town of Plovdiv (Bulgaria)
The present study is a small part of a program for application the methods of passive and active phytomonitoring with herbaceous species, trees, mosses and lichens for assessment of the anthropogenic factor in urban cond...
Micropropagation of Helichrysum italicum (ROTH) G. Don – a medicinal plant with ornamental value
Helichrysum italicum (Roth) G. Don is a Mediterranean vegetal species from the Asteraceae family. The ornamental value of the flowers and the properties of its essential oils contribute to its popularity. The aim of the...
What is known about Cyanoprokaryota and the algal blooms along the Bulgarian Black Sea coast: an overview
This article presents a summary of the research related to the taxonomic composition, algal blooms and toxic potential of representatives from Cyanoprokaryota (Cyanobacteria, Cyanophyta) in the coastal waters of th...
Low temperature extraction of essential oil bearing plants by liquefied gases. 7. Seeds from cardamom (Elettaria cardamomum (L.) Maton)
The chemical composition of extract from the seeds of cardamom (Elettaria cardamomum (L.) Maton, obtained by extraction with tetrafluoroethane was analyzed using GC and GC/MS. The major compounds (concentration higher th...
Milk protein tailoring to improve functional and biological properties
Proteins are involved in every aspects of life: structure, motion, catalysis, recognition and regulation. Today's highly sophisticated science of the modifications of proteins has ancient roots. The tailoring of proteins...