Qualitative Determination of Citalopram and Escitalopram in whole human blood using liquid- liquid extraction and GC/GC-MS
Journal Title: IP International Journal of Forensic Medicine and Toxicological Sciences - Year 2018, Vol 3, Issue 4
Abstract
Citalopram is a antidepressant drug generally prescribed for the treatment of major depression It comes under the SSRIs class selective serotonin reuptake inhibitor It is frequently used offlabel to treat anxiety panic disorder obsessive compulsive disorder premenstrual dysphoric disorderAs per forensic point of view citalopram which is non tricyclic antidepressant is generally prescribed for treating depression because of its undesirable side effects than classical tricyclic antidepressants so they are nowadays more commonly used as the drugs of first choice and therefore the chance of detecting it in the biological materials analyzed in toxicology division is highPresent study has been structured with an aim to test citalopram present in blood and the comparison of citalopram and escitalopram which are the enantiomers For extraction of citalopram from blood sodium tungstate method was used followed by liquid liquid extractionThe alkaline extract of blood obtained from Sodium Tungstate was used for identification by color tests Citalopram is basic in nature but it does not respond to commonly used color test for benzodiazepinesalkaloids Hence various color tests were performed which were reported in literature out of which marquis mandelin gives positive result cobalt thiocyanate has also given positive test for presence of citalopram which can be used in future for testing citalopramFurther the identification of the drug was carried out by thin layer chromatography by various solvent systems In search for different solvent systems various combinations of solvents were tried and reproducible results were produced For the separation of citalopram Escitalopram solvent system Toluene Acetonitrile methanol has given the good Rf values and reproducibleResults Other instrumentation technique like UV visible spectroscopy Gas liquid chromatography GLC GCMS was also used for the qualitative analysis Keywords Citalopram Escitalopram Extraction Tricyclic antidepressants
Authors and Affiliations
Sneha Yadav
Keywords
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- EP ID EP486745
- DOI 10.18231/2456-9615.2018.0020
- Views 100
- Downloads 0
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