Qualitative Histological Analysis of Adrenal Cortex Following Application of Medroxyprogesterone Acetate
Journal Title: Macedonian Journal of Medical Sciences (MJMS) - Year 2010, Vol 3, Issue 2
Abstract
Aim. The aim of the investigation was to make a review of the morphological characteristics of the adrenal cortex following the application of high doses of medroxy-progesterone acetate (MPA). Material and Methods. Total of 48 female Wistar rats were divided into four groups. Control group was given saline daily, intra-muscularly, and the other three groups MPA in doses of 7.5, 15.0 and 75.0 mg/kg bw during 30 days. Paraffin sections from adrenal glands were stained according the following methods: hematoxylin-eosin, Azan modified by Heidenhein, Van-Gieson, Kossa-Goldner, Masson and Florentin. Results. Histological analysis demonstrated that MPA caused the following morphological changes in adrenal cortex: more intensive development of stroma; decrease of adrenal cortex thickness; disturbance of spacial organization of adrenocorticocytes in glomerular zone, fascicular zone and reticular zone; atrophic changes of adrenocorticocytes; disappearance of intermediary zone after application of 75.0 mg/kg bw MPA; appearance of microcysts in fascicular and reticular zones; decrease of cortical proliferate dimensions and accessory adrenal glands, disappearance of spongiocytes from their structure and atrophic changes of glomerular cells; decrease of adrenal cortex vascularisation, necrotic changes localized subglomerularly and in fascicular zone of adrenal cortex. Conclusion. MPA causes atrophic changes in adrenal cortex i.e., the decrease of its cell potential, a phenomenon suggesting that MPA possesses glucocorticoid characteristic.
Authors and Affiliations
Elida Mitevska| Institute of MEP Histology and Embryology, Faculty of Medicine, University “Ss Cyril and Methodius”, Skopje, Republic of Macedonia, Mirko Spiroski| Institute of Immunobiology and Human Genetics, Faculty of Medicine, University “Ss Cyril and Methodius”, Skopje, Republic of Macedonia
Keywords
Related Articles
- EP ID EP8736
- DOI 10.3889/MJMS.1857-5773.2010.0100
- Views 379
- Downloads 19
Non-Foodborne Swine Zoonotic Diseases
The aim of this paper is to give an overview of novel swine non-foodborne zoonotic diseases that have been prominent in the last decade in swine industry. The number of swine present worldwide and the large percentage...
In Vitro Inhibition of Growth and Aflatoxin B1 Production of Aspergillus Flavus Strain (ATCC 16872) by Various Medicinal Plant Essential Oils
The hazardous nature of aflatoxins to human and animals necessitate the need for establishment of control measures. The objective of this study was to evaluate the inhibition of growth and aflatoxin production of Asper...
Observation of Esterase-Like-Albumin Activity during N’- Nitrosodimethyl amine Induced Hepatic Fibrosis in a Mammalian Model
Aim: Hepatic fibrosis (HF) is characterized by irregular growth and amassing of fibrous scar tissues in the liver causing weakened hepatocytes metabolism and protein level alterations, including albumin. Albumin with M...
Calf Muscle (Triceps Surae Complex) Thickness in an Adult Nigerian Population: An Imaging Based Normographic Study
Objective: The thickness of the triceps surae muscle complex was assessed by ultrasound in a young adult Nigerian population. Its relationship with some anthropometric variables and sexual dimorphism was also assessed....
Ocimum Gratissimum Linn Worsens Streptozotocin-Induced Nephrotoxicity in Diabetic Wistar Rats
Effects of ethanolic extract of Ocimum gratissimum leaves (O. gratissimum) on the kidneys in Streptozotocin-induced diabetic rats were studied. Thirty-six adult rats were assigned into six groups (A, B, C, D, E and F)...