QUANTIFICATION OF NIGELLA SATIVA OIL (NSO) FROM BIODEGRADABLE PLGA NANOPARTICLES USING FTIR SPECTROSCOPY
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2014, Vol 6, Issue 10
Abstract
Objective: Quantification of medicinal plant, N. sativa oil, in biodegradable nanoparticles fabricated from PLGA and chitosan was impossible due to the difficulty in the oil extraction-partition method of which the nanoparticle did not dissolve in any solvent.Methods: FTIR method was developed to quantify the loading efficiency of NSO from the intact nanoparticles without the need to solubilise the nanoparticles and extract the NSO thereafter. Beer-Lambert law was applied in the quantification following selection of a few wave number combinations.Results: The method exhibited linearity in the range NSO/PLGA=5-150% with R2=0.9911, RSD=1.68%, LOD=0.89% and LOQ=2.68%. NSO-PLGA nanoparticles revealed complete encapsulation of NSO (loading efficiency=101.5±2.2%) while chitosan-NSO-PLGA nanoparticle showed lower loading efficiency (84.5±1.7%) due to presence of the hydrophilic polymer, i. e. chitosan. This method is fast and easy to apply and does not require sample processing. The method will help to accelerate and improve routine characterisation of NSO nanoparticles during development and optimisation stage.Â
Authors and Affiliations
Abd Almonem Doolaanea, Ahmad Fahmi Harun, Farahidah Mohamed
Keywords
Related Articles
- EP ID EP579437
- DOI -
- Views 69
- Downloads 0
GC-MS BASED CHEMICAL PROFILING AND EVALUATION OF ANTIOXIDANT POTENTIAL OF LEAVES AND STEMS OF ALTERNANTHERA SESSILIS RED FROM SABAH, MALAYSIA
Objectives: The aim of the current study was to perform phytochemical screening and determination of total phenolics, total flavonoids and antioxidant activity of various crude extracts of Alternanthera sessilis red leav...
AN ANTIMICROBIAL PHTHALATE DERIVATIVE FROM BACILLUS CEREUS, THE SYMBIOTIC BACTERIUM ASSOCIATED WITH A NOVEL ENTOMOPATHOGENIC NEMATODE, RHABDITIS (OSCHEIUS) SP
Objective: To isolate and identify the bioactive metabolites from the culture filtrates of a bacterium (Bacillus cereus) symbiotically associated with a novel entomopathogenic nematode Rhabditis (Oscheius) species.Method...
BRIEF REVIEW OF SPECTROPHOTOMETRIC METHODS FOR THE DETECTION OF TETRACYCLINE ANTIBIOTICS
Antibiotics are one of the most common pharmaceutical products, used for the treatment of bacterial ,fungal and parasitic infections. Among antibiotics, tetracyclines are extensively used in both human and animal welfare...
IDENTIFICATION OF LEAD COMPOUNDS WITH COBRA VENOM DETOXIFICATION ACTIVITY IN ANDROGRAPHIS PANICULATA (BURM. F.) NEES THROUGH IN SILICO METHOD
Objective: To validate the cobra venom detoxification activity in Andrographis paniculata and identification of lead molecules.Methods: The structures of phytochemicals were procured from databases or created by ChemSket...
ASSESSMENT OF TWO OR MORE ANTIBIOTICS REGIMEN VERSUS ONE OR NO ANTIBIOTIC REGIMEN IN POST-OPERATIVE SURGERY
Objective: Surgical site infections [SSI] are the leading cause of hospital-acquired infections. The objective of this study was to evaluate whether surgical site infections can be reduced with two or more antibiotics re...