Quantitative Analysis of Adrenal Cortical Histological Alterations After Application of Medroxyprogesterone Acetate
Journal Title: Macedonian Journal of Medical Sciences (MJMS) - Year 2010, Vol 3, Issue 4
Abstract
Aim. The aim of our investigation was to make a quantification of the changes being registered by a quantitative histological analysis of adrenal cortex after long-term application of high doses of medroxyprogesterone acetate (MPA). Material and Methods. A total of 48 female Wistar rats were divided into four groups. The control group was given saline intramusculary, every day, and the other three groups MPA in doses of 7.5, 15.0 and 75.0 mg/kg bw, respectively, during 30 days. Paraffin sections from adrenal glands were stained according the following methods: hematoxylin-eosin, Azan, modified by Heidenhein, and Masson. Results. Gravimetric and variance analysis demonstrated the most remarkable decrease of the adrenal gland weight after the application of 75.0 mg/kg/bw MPA (from 35.66 ± 11.78 to 13.41 ± 8.41 mg (p<0.001). On the other hand, morphometric analysis demonstrated that adrenal cortex volume was the most significantly reduced from 11.30 ± 3.53 in the control group to 2.04 ± 0.90 mm3 in the group treated with 15.0 mg/kg bw MPA (p<0.0001). Adrenal cortex thickness was reduced from 846.06 ± 6.48 to 349.55 ± 4.84 μm after the application of 75.0 mg/kg bw (p < 0.001). After the application of the same dose the glomerular zone thickness was decreased from 48.02 ± 0.44 to 41.40 ± 0.43 μm (p < 0.001), the fascicular zone thickness from 480.72 ± 3.43 to 194.32 ± 2.80 μm (p <0.001) and the reticular zone thicknes reduced from 317.47 ± 4.80 to 113.85 ± 2.28 μm (p < 0.001). The volume of glomerular zone cell nuclei decreased most remarkably from 94.79 ± 0.96 μm3 after the application of 75.0 mg/kg bw to 60.12 ± 0.88 μm3 (p < 0.001). The volume of fascicular zone cell nuceli decreased from 166.30 ± 1.53 to 52.47 ± 0.89 μm3 (p < 0.001), while the volume of reticular zone cell nuceli reduced from 95.61 ± 1.03 to 47.68 ± 0.92 μm3 (p < 0.001). The analysis by means of χ2 test showed that the appearance of tissue necrotic changes in adrenal cortex had a statistical significance only after the application of the highest dose of MPA (p < 0.012). Conclusion. The quantitative histological analysis showed significant decrease of the adrenal cortex, i.e. decrease of glomerular, fascicular and reticular zone thickness and significant decrease of the adrenocorticocytes nuclei volume, changes which suggested that MPA caused an atrophy of the adrenal cortex.
Authors and Affiliations
Elida Mitevska| Institute of MEP Histology and Embryology, Faculty of Medicine, University “Ss Cyril and Methodius”, Skopje, Republic of Macedonia, Mirko Spiroski| Institute of Immunobiology and Human Genetics, Faculty of Medicine, University “Ss Cyril and Methodius”, Skopje, Republic of Macedonia
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- EP ID EP8768
- DOI 10.3889/MJMS.1857-5773.2010.0122
- Views 354
- Downloads 15
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