QUANTITATIVE ESTIMATION OF DRUGS AND PHARMACEUTICALS USING CHLORAMINE-T AND SAFRANIN-O DYE BY AN OXIDATIVE SPECTROPHOTOMETRIC METHOD
Journal Title: International Journal of Pharmaceutical Sciences and Research (IJPSR) - Year 2019, Vol 10, Issue 7
Abstract
Simple, sensitive and selective methods are developed for the spectrophotometric determination of drugs, viz., Cetirizine hydrochloride, Duloxetine hydrochloride, Esmolol hydrochloride, Gemifloxacin, Moxifloxacin and Phenylephrine Hydrochloride based on their reactivity towards Chloramine-T (ChL-T). The method is based on the oxidation of drugs by ChL-T (Excess amount) in presence of acidic medium and estimating the amount of un-reacted ChL-T by Safranin-O (SO) dye at λmax 524 nm. The amount of ChL-T reacted is equal to the drug concentration in this method. The concentration of ChL-T is 10 µgmL-1 and Safranin-O Dye is 10 µgmL-1. The calibration curves obeyed Beer’s law over the concentration range of 2.5-17.5 µgmL-1 (CET), 5-35 µgmL-1 (DUL), 12-84 µgmL-1 (ESM), 10-70 µgmL-1 (GEM) & 2.5-17.5 µgmL-1 (MOX). This method has been applied for the determination of drugs in their pure form as well as in tablet formulations. The method has been validated in terms of guidelines of International Conference on Harmonization.
Authors and Affiliations
Venkateshwarlu Gandu et al.
Keywords
Related Articles
- EP ID EP607042
- DOI 10.13040/IJPSR.0975-8232.10(7).3241-49
- Views 111
- Downloads 0
IMPACT OF HYDROTHERMAL ISOLATION METHOD ON THE RECOVERY OF FENUGREEK SEED HEMI-CELLULOSE ANALYZED THROUGH FTIR-DSC-SEM INTERPRETATIONS
Natural Herbal components like seeds mucilages, exudates, gums, polysaccharides, celluloses, hemicelluloses, starch, etc. are now a day’s exploited as excipients. Literature reveals medicinal, nutraceutical and pharmaceu...
DISINFECTANTS, REGULATION AND REQUIREMENTS - GLOBAL OVERVIEW
Surface disinfectant is the multi-billion-dollar global industry with continuous growth opportunities linked to advancements in technology and innovations. However, innovations and new advancements do not always reach co...
A REVIEW ON SOLID LIPID NANOPARTICLES; FOCUS ON EXCIPIENTS AND FORMULATION TECHNIQUES
Nanotechnology has brought about a significant change in the drug delivery system. A plethora of BCS class II drugs are formulated as solid lipid nanoparticles (SLN) due to its poor solubility and bioavailability and has...
DEMENTIA: A NEURODEGENERATIVE DISORDER
Dementia is a cognitive decline which interferes with normal psychological functioning and behavioral patterns. Dementia is as a group of neurodegenerative diseases, which affects about 1 to 4% of the world’s population...
SYNTHESIS, CHARACTERIZATION, AND DNA- BINDING INTERACTION STUDIES OF A NEW MANNICH BASE AND IT’S METAL COMPLEXES
Cyclic imides such as succinimides, maleimides, phthalimide and their derivatives contain an imide ring and a general structure –CO-N(R)-CO- that confers hydrophobicity and neutral nature. Succinimide (pyrolidine 2,5-dio...