RAPHANUS SATIVUS LINN. A NEW ANTINOCICEPTIVE FOR DIABETIC NEUROPATHY IN RATS DETERMINED BY RANDALL SELITTO APPROACH
Journal Title: Asian Journal of Pharamceutical and Clinical Research - Year 2019, Vol 12, Issue 3
Abstract
Objective: The objective of the study was to evaluate the antinociceptive effect of Raphanus sativus Linn. using Randall Selitto method. Methods: Streptozotocin, lard, casein, cholesterol, DL-methionine, yeast powder, quercetin, thiobarbituric acid, 2-nitrobenzoic acid (5, 5, Dithiobis), hematoxylin, and hydrogen peroxide were used. A diet rich in fat content was fed to the animals for a period of 2 weeks. After a stabilization period of 2weeks, the treatment period started and continued for a period of 8weeks. The nociceptive parameters were assessed once a week by Randall Selitto method and hot plate test. After treatment, the animals were sacrificed, and antioxidant parameters were assessed using sciatic nerve homogenate and histopathological analysis of sciatic nerve. Results: Treatment R. sativus extract (RSE 100 mg/kg and 200 mg/kg) appreciably declined the levels of blood glucose in a dose-dependent manner, and it was comparable with standard quercetin. A significant increase in pain threshold levels was observed by the treatment RSE in hot plate method after the 4th week compared to diabetic control, and it was consistent until the end of treatment (p<0.01, p<0.001). In Randall Selitto method RSE produced a significant increase in paw withdrawal threshold after the 4th week compared to diabetic control, and it was consistently increased until the end of treatment. RSE (100 and 200 mg/kg) significantly restored the levels of antioxidant enzymes and decreased lipid peroxidation in a dose-dependent fashion in comparison with the diabetic control group. RSE (100 mg/kg and 200 mg/kg) attenuated the nerve degeneration and axonal swelling along with quercetin. Conclusion: The findings from the current study showed the antinociceptive and antioxidant effect of R. sativus in neuropathic pain in diabetes.
Authors and Affiliations
SAMBIT KUMAR SAHOO, STHITAPRAGNYA PANDA
Keywords
Related Articles
- EP ID EP603920
- DOI -
- Views 122
- Downloads 0
CARDIOPROTECTIVE POTENTIAL OF PLANTS AND PLANT-DERIVED PRINCIPLES – A REVIEW
Cardiovascular diseases (CVDs) are a class of diseases involving heart or blood vessels. Coronary artery diseases include angina, myocardial infarction (MI), stroke, heart failure, hypertensive heart disease, cardiomyopa...
DOCUMENTATION AND ETHNOMEDICINAL KNOWLEDGE ON WILD EDIBLE MUSHROOMS AMONG ETHNIC TRIBES OF NORTHERN ODISHA, INDIA
ABSTRACTObjective: The objective of the present study was to document the diversity of wild edible mushrooms and their ethnomedicinal uses among differenttribes of Northern Odisha through field survey, questionnaire, and...
CHEMICAL COMPOSITION, ANTIBACTERIAL ACTIVITY, AND ANATOMICAL STUDY OF TEUCRIUM POLIUM L.
Objective: The aim of this work is the simplification of the exploitation of medicinal plants that are widely spread in Algeria such as Teucrium polium and search for new biological molecules; first, we performed an anat...
EVALUATION OF DIURETIC ACTIVITY OF PICRIA FEL-TERRAE LOUR LEAVES EXTRACTS
Objective: The aims of this study was to evaluate the diuretic activity of different leaves extract of Picria fel-terrae using n-hexane, ethylacetate and ethanol as solvents. Methods: The diuretic activity of the extrac...
A REVIEW ON SCREENING METHODS OF BREAST CANCER, CANCER BIOMARKERS AND PHYTOCONSTITUENTS AGAINST BREAST CANCER
  A cancer biomarker refers to a substance or process that is used as an indicative the presence of cancer in the body. A biomarker may be a molecule secreted by a tumor or a specific response of the body to the presen...