Rapid detection of dengue virus by multiplex real time PCR at SMS medical college & attached Hospitals, Jaipur
Journal Title: International Journal of Medical and Health Research - Year 2019, Vol 5, Issue 4
Abstract
Objective: To determine the magnitude of Dengue virus in patients presenting with Dengue like illness (DLI) during the postmonsoon period. Materials and methods: A total of 100 blood samples of patients having DLI, attending SMS Medical College and attached hospitals were collected and processed for Dengue RT-PCR during the month of October 2017. Results: Of the 100 patients enrolled in the study, overall 36(36.0%) patients were positive for dengue by RT-PCR were negative. In the group of positive patients, 24 (66.67%) whereas in the group of negative patients, 46 (71.88%) were males, with no statistically significant differences between the two groups. The age group with the highest positivity for Dengue was in adults between 21 and 30 years old (30.55%), followed by juveniles up to 20 years old (22.22%).Fever was seen in almost all cases. In dengue infection, common symptoms included myalgia (25%), joint pain (33.33%), headache (41.67%), and cough (13.89%). Conclusion: In this study, we intended to bring attention on the presence of viral etiologies of febrile illnesses in Rajasthan. Our results suggest that DENV virus should be considered in differential diagnosis of patients with fever. For the laboratory diagnosis of DENV infection molecular assays are more sensitive in the early stages of illness. Moreover in multiplex PCR other agents could also be tested simultaneously thus adding to diagnostic armamentarium leading to rapid and early diagnosis and management of febrile illnesses.
Authors and Affiliations
Nagender Singh Jhajhria, Bharti Malhotra, Pratibha Sharma, Madhavi Vyas
Keywords
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