Rapid Micropropagation of Androgrphis paniculata Nees, from Nodal Explants to Study Effect of Various Plant Growth Regulators
Journal Title: Indian Journal of Scientific Research - Year 2017, Vol 7, Issue 2
Abstract
Andrographis paniculata Nees, commonly called as ‘kalmegh’ or ‘chiraita’ is frequently used in traditional medicine. Due to extensive wild collection and lack of awareness for the conservation of medicinal plants, many medicinal plants of Chhattisgarh are falling under the category of endangered species. There is an urgent need for the conservation and commercial production of rare and medicinally important plants. The present work is to develop an efficient protocol for the rapid micropropogation of Andrographis paniculata from the nodal segments, with various plant growth regulators. When MS was medium supplemented with BAP, shoot/explants and bud break percentage at concentration of 1.5 mg/l of BAP was found to be 22.25 ± 2.99 and 91.75 ± 3.94 respectively. At 0.5 mg/l concentration of BAP average shoot length, nodes/shoot and leaves/shoot was observed to be 1.875 ± 0.25, 3.25 ± 0.95 and 6.50 ± 1.91 respectively. When MS medium was fortified with Kinetin, at concentration of 2.0 mg/l, shoot/explants and bud break percentage was 25 ± 6.25 and 72.25 ± 9.10 respectively. Similarly, average shoot length nodes/shoot and leaves/shoot at concentration 0.5 mg/l of Kinetin were 1.56 ± 2.49, 2.25 ± 0.95 and 4.5 ± 1.91 respectively. Effect of BAP in combination with Kinetin was further studied. The concentration of BAP was fixed at 1.0 mg/l with various concentration levels of Kinetin. 94.75 ± 2.21 was found to be the bud break percentage both at BAP 1.0 mg/l + Kn 2.0 mg/l and BAP 1.0 mg/l + 2.5 mg/l Kn. Shoot/explants, average shoot length, nodes/shoots and leaves/shoots was found to be 31.50 ± 2.64, 4.37 ± 0.40, 5.75 ± 0.50 and 11.50 ± 1.00 respectively. Among all the growth regulators tested BAP in combination with Kinetin gave best results at concentration of 1.0 mg/l BAP and .05 mg/l of Kinetin.
Authors and Affiliations
S. PANDEY
Keywords
Related Articles
- EP ID EP370079
- DOI -
- Views 99
- Downloads 0
TELEOMORPHS OF Sphaerotheca Fuliginea ON CUCURBITS IN ROHILKHAND REGION OF UTTAR PRADESH (INDIA)
Anamorphs of powdery mildew on cucurbits occur annually throughout the Rohilkhand region of Uttar Pradesh (India). There have been only few published reports on the occurrence of teleomorphs on cucurbits in various state...
SCREENING OF ESSENTIAL OILS OF ANGIOSPERMIC PLANTS FOR THEIR FUNGITOXICITY AGAINST Alternaria alternata FR. KEISSLE
Fresh parts of angiospermic plants were collected from different places of the local area and were subjected to hydrodistillation through Clevengers apparatus so as to isolate the volatile plant products (essential oils)...
EFFECT OF MAGNETIC CLOUD ON COSMIC RAY INTENSITY AND GEOMAGNETIC FIELD IN ASSOCIATION WITH CORONAL MASS EJECTION AND INTERPLANETARY SHOCKS
The relationship between magnetic cloud and Coronal Mass Ejection is a part of research in this field. The view that all ICMEs would originate as flux ropes while only one-third of CMEs show clear magnetic cloud signatur...
VARIATION IN CALORIFIC VALUE OF SEEDS OF Adhatoda vasica FOUND AT DIFFERENT SITES IN AND AROUND BHOPAL MADHYA PRADESH
Adhatoda vasica is one of the medicinal plants of immense medicinal value. This plant is useful in asthma, bronchitis, chronic cough, controlling pain, inflammation and cold etc. The present work is based on detailed st...
MHD FREE CONVECTION FLOW THROUGH A POROUS MEDIUM BOUNDED BY AN INFINITE VERTICAL PLATE WITH CONSTANT HEAT FLUX
This paper considers the problem of free convective flow of a viscous incompressible fluid through a porous medium bounded by an infinite vertical porous plate in the presence of transverse magnetic field. The plate is s...