Real Time Cell Analysis of Model Target Cell Lines Exposed to Purified Lipoprotein (a)
Journal Title: Journal of Advances in Medicine and Medical Research - Year 2016, Vol 16, Issue 4
Abstract
Lipoprotein (a) [Lp(a)] is a novel independent cardiovascular risk factor and it includes, beyond apoB100, apolipoprotein (a), whose molecular weight is dependent on the number of genetically encoded kringle IV type 2 repeats and inversely related with Lp(a) plasma concentration. Risk thresholds for molecular weights have been proposed, but there is not a full consensus and the role of the different isoforms in pathogenesis has not yet been clarified. The aim of the present work is to explore the biological effect of low and high molecular weight Lp(a) isoforms on cultured cells. Real-time impedance analysis has been performed on model cell lines of atherogenesis and Lp(a) metabolism (THP-1, HUVEC, HASMC and HepG2) using affinity purified Lp(a) with 22 (low number) and 31 (high number) kringle IV type 2 repeats, respectively. Normalized Cell Index data show that all the cell lines tested are modified by Lp(a), though with a variable intensity. Low and high molecular weight Lp(a) isoforms at similar concentrations can exert opposite modifications on the impedance kinetics of different cell lines. These data suggest that purified Lp(a) can modify the behaviour of adherent cell lines, an effect which can be detected as impedance variation and which is influenced by its specific isoform.
Authors and Affiliations
Alice Santonastaso, Claudia Scotti
Keywords
Related Articles
- EP ID EP341326
- DOI 10.9734/BJMMR/2016/26869
- Views 86
- Downloads 0
Prevalence and Distribution of Wolff-Parkinson-White Pattern or Syndrome among Young Adult Population in Nigeria
Aims: This study aimed to determine the pattern and distribution of Wolff-Parkinson-White (WPW) pattern or syndrome among young adults. Study Design: This is a cross-sectional descriptive study. Place and Duration of Stu...
Correlation of Body Composition with Pulmonary Function in Adults
Context: General population has a wide variation in physical activities ranging from the people with sedentary lifestyle to people involved in regular physical activity; there is also variation amongst different age grou...
Health-related Microbial Quality of Drinking Water in Kangavar, Western Iran
Evaluation of the microbial quality of drinking water can prevent the water-borne diseases outbreak that is one of the most important challenges in the world. Therefore, the aim of this study was to assess the seasonal v...
The FITT Principle in Individuals with Type 2 Diabetes: From Cellular Adaptations to Individualized Exercise Prescription
Diabetes mellitus is a complex disease that affects millions of people worldwide. Type 2 diabetes (T2DM) arises from a combination of genetic susceptibility and environmental factors, including physical inactivity and po...
Vaginal Candidiasis Infection among Pregnant Women in Aba, Abia State, Nigeria
Epidemiological monitoring of vaginal candidiasis infection associated with preterm delivery and death of the infants is highly desirable especially on pregnant women. The objectives of this study were to determine the p...