Recent Advances in Structure-Based Protein Engineering
Journal Title: Scholars Journal of Applied Medical Sciences - Year 2015, Vol 3, Issue 3
Abstract
The last decade has seen an exponential increase of protein structures solved by X-ray crystallography, NMR and cryo-electron microscopy. The existing information on the protein crystal structure and various computational design toolboxes are furnishing protein engineering more accurately than ever. Structure-based protein engineering involves the application of structural knowledge and software tools to alter protein structures and functions. Much work has been focused on enzyme structure analysis by computational tools to detect key residues responsible for specific properties. We observe that structure-based engineering techniques are potential and compatible approaches that greatly simplify the process of improving certain properties of enzymes. The molecular modeling and de novo design tools are instrumental in increasing antibody stability and binding affinity for therapeutics. They are also proven helpful in identifying epitope of antigens for vaccine development. As the recent advances in structure-guided protein engineering are summarized in the review, it is clear that the structure-based protein engineering could result in tremendous saving in time and investment compared to traditional methods. Keywords: rational design, computational design, enzyme engineering, epitope prediction, antibody engineering.
Authors and Affiliations
Mohammad Mizanur Rahman, Indrani Sarker, Ahmed H. Elmenoufy, Hagar S. Bahlol, Shahriar Zaman
Keywords
Related Articles
- EP ID EP373279
- DOI -
- Views 52
- Downloads 0
Find Out the Association of Serum Mg Levels with Diabetes and Its Comparison with Non-Diabetics
Abstract: The present study was conducted in 100 control and 100 cases who were admitted under Medicine Department /Medicine OPD or Superspeciality Dept. of Endocronology & Neurology in Index Medical College Hospital and...
Therapeutic benefits of carica papaya leaf extracts in dengue fever patients
Carica Papaya (C. papaya) is commonly called as paw-paw and it belongs to the family Caricaceae. It have been successfully employed as a folk medicine for many years for the treatment of dengue infections with haemorrhag...
Rare case of Primary Tuberculosis of Penis mimicking malignant ulcer
Abstract: In literature very few cases of primary tuberculosis of penis has been reported. It is rare even in developing countries like India where pulmonary tuberculosis is common. We report a case of a 61 years old mal...
Association of serum uric acid an independent risk factor in acute ischemic stroke
The aim was to study the association between serum uric acid and acute ischemic non embolic stroke. 1oo patients of first ever life time acute ischemic stroke admitted in D. Y. Patil hospital were included. The blood sam...
Bacterial Isolates and Their Antibiotic Susceptibility Pattern of Uropathogens in Outpatients and Inpatients with Urinary Tract Infection in Tertiary Care Centre, Bikaner - Implications on Empiric Therapy
Urinary Tract Infections (UTI) are the common infections after the respiratory tract infections. The present study was designed to isolates the bacterial uropathogens and their antibiotic susceptibility pattern in outpat...