Red Blood Cell Membrane Physiology In Atorvastatin Treated Rats As Evaluated By Osmofragility Test And Possible Protective Role of Coenzyme Q 10
Journal Title: IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) - Year 2017, Vol 16, Issue 4
Abstract
Background: Erythrocyte Osmotic fragility (EOF) is defined as the degree of hemolysis that occurs when RBC are exposed to osmotic stress. EOF depends upon various factors including the RBC cell membrane composition, integrity, cell size, and shape and surface-volume ratios. Objectives of Study: The present study was conducted to determine the red blood cell membrane physiology in atorvastatin treated rats as evaluated by erythrocyte osmofragility test &, evaluate possible protective role of Co-enzyme Q 10 against atorvastatin induced erythrocyte cell membrane injury. Subjects And Methods:An experimental study was conducted at Animal house, Isra University Hyderabad and Department of Animal Husbandry and Veterinary Sciences Sindh Agriculture University Tando Jam. 50 albino Wistar rats was selected randomly according to inclusion and exclusion criteria. The rats were randomly divided into 5 groups namely A (controls), and experimental groups B, C, D and E. Atorvastatin and Co enzyme Q 10 were given for 6 weeks duration. Erythrocyte osmotic fragility test was performed with Na Cl solutions of difference osmotic concentrations e.g. as 0.1 N NaCl solution, 0.2 N NaCl solution, and so on. Data was analyzed on SPSS version 21.0 (IBM, incorporation, USA). Continuous variable weight was analyzed using students t-test. % Hemolysis was presented as graphs in Microsoft excel sheet. Statistical significance was taken at p≤ 0.05. Results: Experimental rats showed >90% hemolysis at NaCl concentrations of 0.45% and > 95% hemolysis at 0.35% and 0.30% NaCl concentrations. Hemolysis due to osmofragility was noted in atorvastatin treated animals- Groups B and C. Hemolysis was reduced in atorvastatin groups D and E which were treated concomitantly with Coenzyme Q 10. The oral use of CoQ 10 showed a decrease in osmofragility in atorvastatin treated animals. Hemolysis was noted more in all experimental rats compared to controls and hemolysis showed differences in low and high dose atorvastatin treated rats. Conclusion: The present study reports increased osmofragility of red blood cells with atorvastatin. Concomitant Co-enzyme Q 10 administration reduced the osmofragility of red blood cells.
Authors and Affiliations
Dr Humaira Bugti, Dr Navaid Kazi, Dr Ghulam Shah Nizammani, Dr Arsalan Ahmed Uqaili, Dr Salman Ahmed Kazi, Dr Samreen Bugti
Keywords
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