Red Pigment Production By Monascus Purpureus In Stirred-Drum Bioreactor
Journal Title: Science Heritage Journal | Galeri Warisan Sains (GWS) - Year 2017, Vol 1, Issue 1
Abstract
Several studies has been conducted to economically cultivate the Monascus sp. However, the potential of using stirred drum bioreactor in solid state fermentation (SSF) for Monascus sp. cultivation has been relatively understudied. Oil palm frond (OPF) petiole has been used as a potential substrate due to its nutritional contents and to add more value to local agricultural waste. This study reports the production of red pigment by Monascus purpureus FTC 5357 in a 2.3 L bench top – stirred-drum bioreactor. The fungus was grown on moistened OPF substrate (60 % (w/w)) supplemented with 2% (w/w) of soy meal peptone. The effects of different aeration rates (0.3-1.0 vvm of humidified air), agitation programme (4-8 cycles per day), and substrate load capacity (25-40 % (v/v) of total drum capacity) on red pigment production are reported. Aeration rate showed a positively correlated interaction to red pigment production in which the highest red pigment were produced using1.0 vvm (6.09 AU/g dry solid), and non-aerated culture showed the lowest red pigment production (0.81 AU/g dry solid). The agitation programme was also showing the positive trend of interaction, in which 8 cycles per day showed the highest red pigment production (4.34 AU/g dry solid) and 4 cycles per day agitation showed the lowest red pigment production. The red pigment production was peaked at 30% (v/v) drum loading capacity (5.61 AU/g dry solid) and the lowest at 25% (v/v) (0.89 AU/g dry solid), whereas 40% (v/v) substrate capacity was incapable of being mixed due to low power output of agitating motor. Results suggested that OPF was a potent source of substrate for the cultivating Monascus purpureus using SSF and all 3 factors (aeration, substrate load capacity and agitation programme) were significantly influenced the red pigment production.
Authors and Affiliations
Mohamad Al Aamin Razali, Farhan M. Said
Keywords
Related Articles
- EP ID EP255951
- DOI 10.26480/gws.01.2017.13.15
- Views 128
- Downloads 0
Lactic Acid Bacteria With Antimicrobial Properties Isolated From The Instestines Of Japanese Quail (Coturnix Coturnix Japonica)
Lactic acid bacteria (LAB) confer many advantages to humans and animals and they provide natural protection against bacterial pathogens such as Salmonella typhymurium and Eschericia coli. LAB strains have been isolated f...
HIGH-QUALITY DNA FROM PEAT SOIL FOR METAGENOMIC STUDIES: A MINIREVIEW ON DNA EXTRACTION METHODS
The understanding of microorganism’s biodiversity in the peatland soil through non-cultivation based approach provide important inputs towards prediction of the ecosystem response towards the changing environment. The ch...
IN-VITRO REGENERATION OF ORTHOSIPHON STAMINEUS (MISAI KUCING) USING AXILLARY BUD
Orthosiphon stamineus or known as Misai kucing is a popular herbal tea plant that helps in treating the ailments of kidney and bladder, diabetes mellitus and gout. Due to high demand of this medicinal plant, a large prop...
Study of cucumber mosaic virus gene expression in capsicum annuum
Cucumber mosaic virus (CMV) is a plant pathogenic virus in the genus Cucumovirus, family Bromoviridae. It has the potential and reputation of having the widest host range of any known plant virus including monocotyledons...
ANTIOXIDANT AND CYTOTOXIC EFFECTS OF CURCUMA MANGGA AND BOSENBERGIA ROTUNDA ETHANOLIC EXTRACTS ON MCF-7 CANCER CELL LINES
Curcuma mangga and Bosenbergia rotunda which belongs to Zingiberaceae family are largely distributed in South East Asian countries including Malaysia. These plants have been traditionally used for treatment of various di...