Study of cucumber mosaic virus gene expression in capsicum annuum
Journal Title: Science Heritage Journal | Galeri Warisan Sains (GWS) - Year 2017, Vol 1, Issue 2
Abstract
Cucumber mosaic virus (CMV) is a plant pathogenic virus in the genus Cucumovirus, family Bromoviridae. It has the potential and reputation of having the widest host range of any known plant virus including monocotyledons and dicotyledons, herbaceous plants, shrubs and trees. CMV is one of the major diseases in Capsicum annum (chilli). Chilli plant samples exhibiting virus-like disease symptoms were collected from Taman Pertanian Indera Mahkota (location 1) and Greenhouse 12 of Horticulture Research Centre, Serdang (location 2). Viral disease was detected based on symptoms like mosaic-mottling, yellow ringspots and cholorotic that appeared on the leaves. The isolation of total RNA was done by using Vivantis GF-1 total RNA extraction kit. RT-PCR technique was used to detect the presence of virus disease symptoms gene in chillies. Identification of causal agents was based on cDNA amplified product size, using virus-specific oligonucleotides. Actin was used as the internal PCR control. The product size of the DNA fragment was 315 bp. From RT-PCR, the expression of CMV can be detected in chilli plants that exhibited the virus-like disease symptoms. This research revealed that some of the chilli plants at the Taman Pertanian Indera Mahkota and Greenhouse 12 of Horticulture Research Centre have been affected by this viral disease.
Authors and Affiliations
Nur Hafizah Azizan, Zulkifli Ahmad Zainal Abidin, Ing Chia Phang
Keywords
Related Articles
- EP ID EP257013
- DOI 10.26480/gws.02.2017.29.31
- Views 155
- Downloads 0
RESEARCH ON MENTAL HEALTH STATUS AND THE RELATIONSHIP BETWEEN SPIRITUAL BELIEF AND SELF – HARMONY
According to the questionnaire survey of 500 graduate students on mental health, spiritual belief and self-harmony, through mathematical statistical analysis, it was found that :(1) overall, the psychological status of g...
ANTI-DIABETIC POTENTIAL OF PEPTIDE FROM P. NIRURI REVEALS THROUGH CARBOHYDRATE HYDROLYZING ENZYME INHIBITION ASSAY
Herb Phyllanthus niruri (P. niruri) is known to have various pharmacological functions including anti-diabetic properties. In this research, protein extracts from different plant parts (leave, fruit and stem) of P. nirur...
Salicylic Acid Mitigates Pb Stress In Nicotiana Tabacum
The exposure of plants to Pb stress increases the generation of reactive oxygen species (ROS) including O2–, H2O2 and ¯OH. ROS are highly reactive substances that capable of altering normal cellular metabolism via oxidat...
DIVERSITY OF BIVALVES IN MANGROVE FOREST, TOK BALI KELANTAN, MALAYSIA
A study on the diversity and some ecological aspects that related to the abundance of infaunal bivalve species was done at Tok Bali mangrove, Kelantan, Malaysia. Samples and data collection was conducted during three dif...
THE POTENTIAL OF DRIED AND FRESH EXTRACTS OF SANSEVIERIA TRIFASCIATA TO MITIGATE ALEXANDRIUM TAMIYAVANICHII, A TOXIC DINOFLAGELLATE
Dinoflagellate is the second largest group of phytoplankton in the marine environment and many of the species have been recognized as toxic species. The high abundance of this species can cause a lot of problems to human...