Relation of Sirtuin 1 Gene Polymorphisms with Lipid Profile in Hemodialysis Patients
Journal Title: Biotechnology Journal International - Year 2014, Vol 4, Issue 8
Abstract
Very little is known about the genetic variation of SIRT 1 and its effects on energy homeostasis in humans. Mammalian SIRT1deacetylates a host of target proteins that are important for apoptosis, the cell cycle, circadian rhythms, mitochondrial function and metabolism. In particular, much current research focuses on the impact of SIRT1 in glucose homeostasis, lipid metabolism and energy balance. Objective: To study the relationship of sirtuin 1 gene polymorphisms with lipids profile in hemodialysis patients. Subjects and Methods: This study included 70 Egyptian subjects (45 patients on hemodialysis and 25 age and gender matched healthy control group). The genotyping of SIRT1 rs7895833 in the promoter region, rs7069102 in intron 4, and rs2273773 in exon 5 was performed using polymerase chain reaction with confronting two-pair primers assay (CTPP). Serum TC, TG, HDLc, LDLc, fasting glucose, urea and creatinine were measured by standard colorimetric methods. Results: The patients had higher diastolic and systolic BP (P<0.001), fasting blood glucose (P<0.001), TC (P<0.001), TG (P=0.006), LDLc (P=0.004), urea (P<0.001) and creatinine (P<0.001). Males and female patients differ according to cause of hemodialysis (P= 0.02) and serum creatinine (P=0.007). Control subjects of sirtuin1 rs7895833 showed significant A allele compared with patients (46% vs. 27.78% P= 0.04) while C allele of sirtuin1 rs7069102 not differ between groups (P>0.05). Sirtuin 1 rs2273773, patients showed significant lower frequency of C allele compared with control (26.67% vs.44% P= 0.04,). Conclusion: Significant association of SIRT1 rs7895833 and rs2273773 polymorphisms with dyslipidemia and blood pressure may modulate disease course in hemodialysis.
Authors and Affiliations
Safaa Tayel, Yasser Elghobashy, Abeer ALrefai, Said Khamis, Hany Elbarbary
Keywords
Related Articles
- EP ID EP237715
- DOI 10.9734/BBJ/2014/12326
- Views 118
- Downloads 0
Phytochemical Screening and Antimicrobial Activity of Bryophyllum pinnatum Extracts
The hexane, ethyl acetate and methanol extracts of the wood and stem bark of Bryophyllum pinnatum were investigated for their phytochemical constituents and activity against selected microorganisms. Phytochemicals found...
Comparative Studies on Some Edible Oils for Biodiesel Production in Nigeria
With the growing population and subsequent increasing demand for energy in Nigeria, coupled with the under-developed electrical energy platforms and the vast problems of the convectional energy sources such as continuous...
Exploration of Phytochemical and Antibacterial Potentiality of Anagallis arvensis L. Extract against Methicillin-Resistant Staphylococcus aureus (MRSA)
Context: Anagallis arvensis L. (Scarlet pimpernel) was used to treatment of several ailments in several countries. Objective: The aim of this study was to evaluate the in vitro antimicrobial activity of leaf methanolic e...
Potentialities of Yeast Strains to be Used as Freeze-Drying Starters for the Production of Traditional Sorghum Beer in Côte d’Ivoire
Freeze-drying is a well-known dehydration method widely used to preserve microorganisms. In order to produce freeze-dried yeast starter culture for the brewing purpose of African traditional sorghum beer, we tested toler...
Mitochondrial Diversity and Time Divergence of Commonly Cultured Cichlids in Nigeria
Aim: The aims are to study the genetic diversity and population structure of Tilapia fish species in Nigeria using mtDNA D-loop region and time divergence of these various groups of fish to give a baseline information f...