Relationship Between Age, CD14+ Leukocytes And Inflammatory Cytokines In Indonesian Children With Recurrent Tonsillitis
Journal Title: IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) - Year 2017, Vol 16, Issue 11
Abstract
Tonsillitis is common among children but the incidence and inflammatory responses varies according to gender, race, and age of the patients. The present study was designed to determine association strength between sex and age with immune response parameters, between expression of CD14 and inflammatory cytokines, and between cytokines molecules IFN-γ and TNF-α in Indonesian children with recurrent tonsillitis. Following informed concent, venous blood of the children (17 boys and 14 girls) were taken and divided into two parts. The first part (1 ml) was subjected to CD14+ leukocytes (lymphocytes, monocytes and neutrophils) analysis by whole-blood flowcytometry-based method. The second part (3 ml) was prepared for serum cytokine measurement using sandwich ELISA method. The results showed that in children with recurrent tonsillitis, the CD14 and cytokines content in their serum is not associated with sex. Furthermore, neither CD14 leukocytes nor serum cytokines correlated with age. However, there was a strong association between CD14 leukocytes with serum cytokines (P<0,031), and between IFN-γ with TNF-α (P<0,001). It can be inferred that in Indonesian children with recurrent tonsillitis, immune response parameters might not determined by sex and age. However, serum cytokines play a role in CD14+ expression, and cytokines themselves, especially IFN-γ and TNF-α can positively predict each other.
Authors and Affiliations
Fatah Satya Wibawa, Eryati Darwin, Irza Wahid, Netti Suharti
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