Relevance of Potential Microorganisms for Enhancing Biogas Production
Journal Title: IOSR Journal of Biotechnology and Biochemistry (IOSR-JBB - Year 2018, Vol 4, Issue 2
Abstract
Bacteria were isolated from the sewage water for the purpose of enhancing biogas production. The sample was serially diluted up to 10-7 dilution, for the isolation of hydrolytic, acidogenic, acetogenic and methanogenic bacteria. However, nutrient agar was used for the isolation of hydrolytic bacteria, while basal media supplemented with specific substrate has been used for the isolation of acidogenic, acetogenic and methanogenic bacteria. After the isolation, the hydrolytic bacteria from the nutrient agar plates were selected on the basis of prominence for the preliminary screening of bacteria. After preliminary screening the efficient strains showed highest zone of clearance were selected for enzyme assay along with acidogenic, acetogenic and methanogenic bacteria. Based on the highest enzyme activity of hydrolytic (cellulase - 21.3U/ml, protease - 205.6U/ml, lipase-23.2U/ml), acidogenic (acetate-520U/ml), acetogenic (acetate dehydrogenase-325.2U/ml), methanogenic (methyl transferase-26.7U/ml) bacteria, the potential bacteria were selected and those were identified as Bacillus subtilis, Pseudomonas stutzeri, Brachybacterium sp. and Clostridium perfringens. Biogas production from poultry waste and cow dung along with four potential bacteria showed a highest methane concentration of 72%, which was relatively higher than that of control (58%). The analysis of biochemical compounds showed that there was a decrease in various compounds, which was also supported by the FTIR spectra obtained however the present study showed an increased biogas production efficiency of 18% was achieved through the addition of microbes, hence such kind approach with microbial addition would enhance biogas production in large extent in the meantime.
Authors and Affiliations
Gopinath. L. R. , Deepa. N. , Divya. D. , Parimala. M, Premalatha. K. , Archaya. S
Keywords
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