Research on a new microtubule technology of reagent bursting for crossmatching test
Journal Title: Chinese Journal of Blood Transfusion - Year 2023, Vol 36, Issue 6
Abstract
Objective To develop a quick and accurate crossmatching test technology without the power equipment and additional reagents before blood transfusion, so as to improve the timeliness and safety of blood transfusion treatment in sudden situations as war or natural disasters. Methods The irregular antibodies quickly promote coagulants (QPC) were used as the reaction medium reagent. The 200 μL QPC were wrapped in the bursts bead and preset within different recess of the detection tubes. The bursts beads were squeezed with the reagent left in the well, then the blood samples were dropped in the main(recipient plasma: 200 μL, donor 3%—5% RBC: 100 μL) and secondary(donor plasma: 200 μL, recipient 3%—5% RBC: 100 μL)reaction grooves. The result interpretated by hand wrestling or 1 500 g centrifugation of 15 seconds. Meanwhile, the comparing experiments with the prior methods were implemented to evaluate the method’s reliability. Results The results of the bursting reagent, being stored at 37℃ for one week, were consistent with those of the freshly prepared cross-matching reagent, indicating that the bursting reagent was practical in the field and had good stability at normal temperature. No statistical difference between the sensitivity and the results of the microcolumn gel method was noticed by paired data t test (P>0.05). The parallel cross matching tests of 50 clinical samples were performed by microcolumn gel method and coagulant-bursting technique; the Kappa value was 0.973 2, and irregular antibodies were detected in 2 cases, with concordance rate at 100%, showing good consistency. Conclusion The improved method is simple and fast, and also safe and reliable for compatibility testing before blood transfusion, which is especially suitable for the field rescue of the wounded in wartime and sudden natural disasters, and is worthy of popularization.
Authors and Affiliations
Yintu MA, Xiaoya YANG, Yiman ZHAO, Li CHEN, Lei GAO, Jingyan ZHOU, Hao YAN, Lihua LI
Keywords
Related Articles
- EP ID EP738562
- DOI 10.13303/j.cjbt.issn.1004-549x.2023.06.005
- Views 81
- Downloads 0
Quality monitoring indicator system in blood banks of Shandong: applied in blood donation services, component preparation and blood supply process
Objective To establish an effective quality indicator monitoring system, scientifically and objectively evaluate the quality management level of blood banks, and achieve continuous improvement of quality management in bl...
Impact of blood screening strategies for Hepatitis C virus on blood safety
Objective To establish a reasonable and effective blood screening strategy for Hepatitis C virus (HCV), so as to reduce the risk of blood transfusion transmission, ensure blood safety and improve the quality of blood scr...
Expert consensus on the resolution level of the methods for HLA genotyping
Human leukocyte antigen (HLA) genotyping has been widely used in establishing the database of the donors for hematopoietic stem cell, HLA matching selection between the donor and recipient, establishing the database of p...
Biological identification and molecular structure model analysis of Bx02
Objective To identify two ABO discrepancy samples and explore the molecular mechanism. Methods The serological phenotype of the proband was determined with standard serological methods. ABO genotype was determined by pol...
Correlation between malignant tumors and ABO blood types in Chaoshan area, Guangdong
Objective To investigate the relationship between ABO blood types and the risk of malignant tumors in Chaoshan area, Guangdong. Methods Chi-square test was used to analyze the distribution of ABO blood types between 45 8...