Research on HLA-A∗ 02 restricted CD8 + T cell immune responses in recovered individuals with COVID-19
Journal Title: Journal of Air Force Medical University - Year 2023, Vol 44, Issue 7
Abstract
Objective To investigate the HLA-A∗ 02 restricted severe acute respiratory syndrome coronavirus 2 (SARSCoV-2) epitope-specific CD8 + T cell responses in peripheral blood of individuals infected with SARS-CoV-2 after 4 5 months of rehabilitation. Methods Based on the previously identified HLA-A∗ 0201 restricted SARS-CoV-2 spike protein CD8 + T cell 9-mer peptide epitopes ALNTLVKQL and YLQPRTFLL, flow cytometry was used to screen a total of 27 HLA-A∗ 02 positive individuals who had received SARS-CoV-2 inactivated vaccines and then infected with SARS-CoV-2 and recovered for 4 5 months after infection. HLA-A∗ 02 / SARS-CoV-2 9-mer peptide tetramers were constructed. Enzyme-linked immunospot assay, tetramer staining and intracellular cytokine staining were used to detect the frequency, phenotype, and ability for cytokines and cytotoxic mediator secretion of SARS-CoV-2 epitope-specific functional CD8 + T cells in the peripheral blood of recovered individuals, respectively. Results SARS-CoV-2 9-mer peptide epitopes ALNTLVKQL and YLQPRTFLL specific CD8 + T cell responses with IFN-γ secretion could be detected respectively in the peripheral blood of HLA-A∗ 02 + recovered individuals, and the frequency of tetramer + CD8 + T cells remained at a high level in the recovered individuals. SARS-CoV-2 epitope-specific CD8 + T cells mainly exhibited the CCR7 - CD45RA - effector memory phenotype and the CD27 - CD28 - highly differentiated phenotype, which could secrete a variety of cytokines such as IL-2, IFN-γ and TNF-α, as well as cytotoxic mediators granzyme B and perforin. Conclusion HLA-A∗ 02 restricted SARS-CoV-2 epitopespecific CD8 + T cell responses can be detected in peripheral blood after recovery from SARS-CoV-2 infection, and its response frequency can last at least 4 5 months after recovery, which still has antiviral functions and effects.
Authors and Affiliations
XUE Manling, XU Xiaoyue, WANG Yuling, ZHANG Xiyue, TANG Kang, ZHANG Yusi, ZHANG Chunmei,ZHANG Yun, ZHUANG Ran, JIN Boquan, MA Ying
Keywords
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