Resistance of Bradyrhizobium sp. (Lotus) to metallic stress
Journal Title: Agropastoralis Scientia - Year 2024, Vol 1, Issue 2
Abstract
The objective of this study was to characterize a bacterial isolate of Bradyrhizobium sp. (Lotus) and to determine its resistance to trace metal elements (TM) such as copper (Cu(II)) and cadmium (Cd(II). Bradyrhizobium sp. (Lotus) was isolated from nodulated plants of Lotus ornithopodioïdes. Different characteristics of the strain were evaluated, such as growth, mobility, enzymes, and polysaccharides production. The minimum inhibitory concentration (MIC) of TM was determined in vitro and in silico by measuring the optical density at a specific wavelength. The isolate was identified as Bradyrhizobium sp. (Lotus), which is a Plant Growth Stress Regulating Bacterium (PGSRB), Gram-negative and slow growing, producing succinoglycan, mobile and possessing urease activity. The isolate produces Indole Acetic Acid (IAA) at 70.6 µg mL-1. Bradyrhizobium sp. (Lotus) isolate showed more pronounced resistance to Cd(II) compared to Cu(II). The MIC of Cd(II) was estimated in silico to be approximately 10 g L -1 , while the MIC of Cu(II) was 2 g L-1. Statistical analyses confirmed a significant relationship between the concentration of TM and the number of bacterial cells. This study allowed the characterization of the bacterial isolate of Bradyrhizobium sp. (Lotus) and determination of its resistance to TM. The results suggested that this strain could be used in bioremediation applications, involving environments contaminated with heavy metals such as copper and cadmium.
Authors and Affiliations
Hoceme DEGAÏCHIA, Ahmed Ramzi BOUSSAHOUA & Touati BAKRIA
Keywords
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Resistance of Bradyrhizobium sp. (Lotus) to metallic stress
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