Resistant Genes blaCTX-M, blaTEM and blaSHV encoding ESBL in surgical site infection causing Escherichia coli and Klebsiella pneumoniae- A report from a tertiary care hospital
Journal Title: IP International Journal of Medical Microbiology and Tropical Diseases - Year 2018, Vol 4, Issue 1
Abstract
Introduction Surgical site Infections SSI rank third among hospital acquired infections worldwide According to the WHO report of 2016 the global burden of SSI ranges from 25 to 415 ESBL producing Ecoli and Klebsiella pneumoniae are frequently being isolated from these infections owing to the high transmission density of plasmidmediated ESBLs in hospital settingAim To survey for antimicrobial resistance encoding genes of ESBL blaTEM blaCTXM and blaSHV among Escherichia coli and Klebsiella pneumoniae isolated from surgical site infections in a tertiary care hospitalMaterials and Methods A cross sectional study was carried out over a period of one year in the department of Microbiology SRM Medical College from March 2016March 2017 A total of 136 pus swabs or pus aspirates were collected from postoperative male and female adult patients Microbiological investigations of Gram staining Culture and biochemical tests were performed with the samples Antimicrobial susceptibility testing for screening and phenotypic detection of ESBL production was carried out according to Clinical Laboratory and Standards Institute guidelines 2016 Conventional Polymerase chain Reaction PCR was performed using specific primers to amplify ESBL encoding genes among ESBL producing Escherichia coli and Klebsiella pneumoniae isolatesResult Out of 136 samples tested 76 5588 Gram negative bacteria were isolated The most common causative agent of SSI was Escherichia coli 30 3947 followed by Klebsiella pneumonia 17 2237 Phenotypic testing by combination disk method detected 40 of Ecoli and 235 of Klebsiella to be ESBL producers They were highly resistant to Ampicillin 86 Cefepime 4693 Ceftazidime 6275 and Ciprofoloxacin 5098 blaTEM was the predominant ESBL gene detected by Conventional PCRConclusion Early and accurate detection of ESBL is essential to reduce the spread of drug resistance among nosocomial bacteriaKeywords Surgical Site infections Escherichia coli Klebsiella pneumoniae Extended Spectrum Beta lactamase PCR resistant genes TEM
Authors and Affiliations
Shaweez Fathima. S, V. Mangayarkarasi, Stephen Pradeep Moses
Keywords
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- EP ID EP485827
- DOI 10.18231/2455-6807.2018.0002
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