Role of Gene Xpert Mtb/Rif in Diagnosis of Tuberculous Pus
Journal Title: INTERNATIONAL JOURNAL OF CURRENT RESEARCH IN MEDICAL SCIENCES - Year 2018, Vol 4, Issue 2
Abstract
Background- Extra-pulmonary tuberculosis (EPTB) remains a crucial challenge for the physicians with respect to timely diagnosis and treatment. The most common appearance of EPTB is tuberculous lymphadenitis with cervical lymphadenopathy being the most frequent. Many EPTB patients present with pus and discharging sinuses from lymph nodes, bones and joints. Objective: To evaluate the performance of GeneXpert for detection of mycobacterium tuberculosis in clinically suspected cases of pus samples. Methods: This prospective study was carried out in Dept. of Pulmonary Medicine, Govt. Medical College, Amritsar, from july 2015 to july 2017 and comprised pus samples of people having lymph node discharging sinuses, empyema, psoas abscess etc. The pus samples were subjected to Ziehl-Neelsen smear microscopy as per World Health Organisation's protocol and GeneXpert as per manufacturer protocol. Role of GeneXpert and Rifampicin resistance were determined in tuberculous pus samples. Results: Out Of the 142 pus samples, 85 (59.8%) were positive on GeneXpert and 38 (26.7%) on Ziehl-Neelsen smear. Rifampicin resistance was detected in 5(5.8%) pus samples by GeneXpert. Conclusion: GeneXpert MTB/RIF assay appears to be an accurate tool for rapid detection of MTB in pus samples. It has a good potential for diagnosis of extrapulmonary tuberculosis in resource-limited settings resulting in an improvement in patients' management and outcome.
Authors and Affiliations
Sandeep Gupta1 , Nirmal Chand Kajal2 , Amaresh Kumar Shukla3 , Amarendra Kumar Shukla4 , Ashi Singh5 , NS Neki6
Keywords
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- EP ID EP552756
- DOI 10.22192/ijcrms.2018.04.02.014
- Views 126
- Downloads 0
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