Role of Tumor Necrosis Factor Alpha α and Interleukin 10 Gene Polymorphisms in Irritable Bowel Syndrome Patients
Journal Title: International Journal of Health Sciences and Research - Year 2017, Vol 7, Issue 3
Abstract
Aims: The current study conducted to determine the frequency of the IL-10 (-1082G/A) and TNF-α (-308G/A) polymorphisms in subjects with IBS in Sudan. Methods: After giving written consent, a total of 71 patients with symptoms of IBS according to the Rome III criteria and 40 controls were enrolled. DNA was extracted from peripheral blood leucocytes of subjects using salting out method. Polymorphisms were determined by PCR-RFLP method. SPSS software program was used for statistical analysis. Results: Patients with irritable bowel syndrome had significantly reduced frequencies of the high producer genotype for interleukin 10 than controls (21% v 32%; p=0.003). there was no significant difference in the frequency of the genotypes or the alleles of the IL-10 (-1082G/A) and the TNF-α (-308G/A) polymorphisms between IBS and the controls, however it was demonstrated that the high producer (AA) TNF α genotype was more prevalent in IBS patients compared to healthy controls (8.5% VS zero). IBS patients were classified as IBS-D (53.5 %), IBS-C (18.3 %) and IBS-A/M (28.2 %) and there were no statistical differences IBS subgroups, were found among these IBS subgroups 57% of patients had family history of IBS. Conclusions: The frequency of the IL-10 (-1082G/A) and TNF-α (-308G/A) genotypes was similar in IBS and controls. However, there was a greater frequency of the low producer of IL-10 in those subjects with IBS-D, suggesting a genetic predisposition to abnormal immune regulation due to a lower anti-inflammatory cytokines IL10 in this subgroup.
Authors and Affiliations
Eltayeb LB
Keywords
Related Articles
- EP ID EP368713
- DOI -
- Views 83
- Downloads 0
Prevalence of Recurrent Aphthous Ulcers in Dental Student: A Questionnaire Based Study
Introduction: The classic clinical presentation of recurrent aphthous ulcers (RAU) is recurrent, self-limiting ulcers that mainly affect non keratinized oral mucosa. They clinically present in 3 different forms, minor, m...
Haematological Effects of Oral Administration of Aqueous Leaf Extract of Moringa Oleifera in Wistar Rats: Further Evidence of Immunomodulatory Potential
Haematological effects of oral administration of aqueous leaf extract of Moringa Oleifera in male wistar rats was investigated in this study. Twenty five (25) wistar rats were used, which were divided into 5 groups of 5...
A Study on Socio Demographic and Fertility Characteristics of Ante Natal Women Attending Urban Health Clinics, In Slum Areas of Hyderabad
Background: In any community mothers and children constitute a priority group. The problems affecting the health of mother and child are multifactorial. This study which was done in urban slums of Hyderabad, facilitates...
Analysis of No Show Patients at Day Surgery Program in a Multidisciplinary 1500 Bedded Hospital
Aims: To identify causes of patients not showing to their day surgery which will help to apply measures to reduce it. Settings and Design: This is a cross-sectional study. All patients who didn’t show on their elective s...
Serum Calcium and Alkaline Phosphatase Level among Pre-Menopausal and Post-Menopausal Women in Rupandehi District of Nepal: A Co-Relational Study
Bone turnover markers and its consequence to poor health outcomes among post- menopausal women have been well understood elsewhere, but it is not well researched in Nepal. Objective: This study is aimed to assess the ass...