Rooting and acclimatization of transgenic papaya plants var. Red maradol
Journal Title: Biotecnología Vegetal - Year 2008, Vol 8, Issue 1
Abstract
The disease caused by Papaya ringspot virus is the most important in papaya worldwide. The use of biotechnological techniques, as auxiliary tools, has facilitated the genetic improvement in papaya. Nevertheless, this species has a lot of constraints, mainly during rooting and acclimatization phases. For that reason we developed the present work. The in vitro and ex vitro rooting was evaluated. Culture media with different concentrations of indol-3-butiric acid hormone were used in the in vitro rooting. The influence of Trichoderma harzianum bioproduct in the acclimatization of plants was also studied. The in vitro rooting of transgenic plants was achieved by applying 2 mg.l -1 of indol-3-butiric acid in the culture medium. The ex vitro rooting with high percentages of plants survival was also obtained. The applications of T. harzianum bioproduct on the substrate, previous to the plantation, demonstrated its stimulating effect.
Authors and Affiliations
Maylin Cruz , Ana L. Darías , Dariel Cabrera , Amado Pérez , Mileidy Cruz-Martín , Tatiana Pichardo , Rafael G. Kosky , Orelvis Portal
Keywords
Related Articles
- EP ID EP683878
- DOI -
- Views 154
- Downloads 0
Obtaining genetic profiles of Stevia rebaudiana Bertoni plants propagated by in vitro culture and cuttings
Stevia rebaudiana Bertoni is worldwide known for its medicinal properties. The objective of this work was to obtain genetic profiles of in vitro grown plants or propagated by cuttings to detect genetic polymorphisms. For...
Effect of different disinfection methods on the in vitro establishment of Guadua angustifolia Kunth
The bamboo is a plant that possesses multiple uses with an enormous economic, social, cultural and environmental impact. The present research work was developed with the objective of determining the effect of different d...
Effect of in vitro culture on the metabolic profile of Psidium guajava L.
The use of in vitro techniques for the production of secondary metabolites in the pharmaceutical industry has increased widely. The organ culture is one of the alternatives for the identification of new compounds or to...
Plant growth regulators in horticulture: practices and perspectives
The plant growth regulators (PGRs) modulate plant growth and development and mediate responses to both biotic and abiotic stresses. This paper aims to expose, reflect and discuss about the practical use of PGRs in hort...
In vitro organogenesis in Digitalis genus
The genus Digitalis have special interest due to the therapeutic properties of its secondary metabolites, known generically as cardenolides. These compounds are commonly used in the treatment of cardiovascular disease....