Rooting and acclimatization of transgenic papaya plants var. Red maradol
Journal Title: Biotecnología Vegetal - Year 2008, Vol 8, Issue 1
Abstract
The disease caused by Papaya ringspot virus is the most important in papaya worldwide. The use of biotechnological techniques, as auxiliary tools, has facilitated the genetic improvement in papaya. Nevertheless, this species has a lot of constraints, mainly during rooting and acclimatization phases. For that reason we developed the present work. The in vitro and ex vitro rooting was evaluated. Culture media with different concentrations of indol-3-butiric acid hormone were used in the in vitro rooting. The influence of Trichoderma harzianum bioproduct in the acclimatization of plants was also studied. The in vitro rooting of transgenic plants was achieved by applying 2 mg.l -1 of indol-3-butiric acid in the culture medium. The ex vitro rooting with high percentages of plants survival was also obtained. The applications of T. harzianum bioproduct on the substrate, previous to the plantation, demonstrated its stimulating effect.
Authors and Affiliations
Maylin Cruz , Ana L. Darías , Dariel Cabrera , Amado Pérez , Mileidy Cruz-Martín , Tatiana Pichardo , Rafael G. Kosky , Orelvis Portal
Keywords
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